anti-carboxyethyllysine rabbit mab

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Recombinant Rabbit Monoclonal Anti-Carboxyethyllysine Rabbit mAb Clone Number: 12H4L5 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB ICC/IF Reactivity: All Synonyms: Kcel Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms Kcel UniProt ID / Immunogen Carboxyethylated lysine peptides MW (kDa) Multiple Specificity Anti-Carboxyethyllysine Rabbit mAb detects proteins post-translationally modified by carboxyethylation on lysine residues. This pan antibody recognizes carboxyethylated lysine independent of its surrounding sequences. Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 All ICC/IF 1:50 Human Properties Purity Protein A and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background N epsilon-(carboxyethyl)lysine (CEL), one of the advanced glycation end products (AGEs), is a stable, nonenzymatic chemical modifications of protein lysine residues resulting from glycation and oxidation. Recently, it has been demonstrated that gut microbiota mediates an aging-associated decline in gut barrier function, allowing AGEs to leak into the bloodstream from the gut and impairing microglial function in the brain. CEL was also detected in human lens proteins at a concentration similar to that of CML(CEL homolog), and increased with age in parallel with the concentration of CML. Although CEL was formed in highest yields during the reaction of methylglyoxal and triose phosphates with lysine and protein, it was also formed in reactions of pentoses, ascorbate and other sugars with lysine and RNase. Cellular location Nucleus, Cytoplasm Images Dot Blot Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Peptide amount: 1: 4ng, 20 ng, 100 ng; 2-10: 100 ng Exposure time: 30 secondsThe list of peptides is included in the table below. WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: (-): HepG2; (+): HepG2 + methylglyoxal (2 mM, 24 hours) + trichostatin A (1 uM, 5 hr)Protein loading amount: 20 μgExposure time: 60 sPredicted band size: MultipleObserved band size: Multiple Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: (-): Rat brain; (+): Rat brain was ground +UV (6mJ/cm2, 2 h), followed by protein extractionProtein loading amount: 20 μgExposure time: 60 sPredicted band size: MultipleObserved band size: Multiple Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: (-): Mouse liver; (+): Mouse liver was ground + UV (6mJ/cm2, 2 h), followed by protein extractionProtein loading amount: 20 μgExposure time: 60 sPredicted band size: MultipleObserved band size: Multiple Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: (-): 1: HeLa, 2: SH-SY5Y, 3: BRL, 4:Neuro2aProtein loading amount: 20 μgExposure time: 60 sPredicted band size: MultipleObserved band size: Multiple Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: Rat liver, 2: Mouse brainProtein loading amount: 20 μgExposure time: 30 sPredicted band size: MultipleObserved band size: Multiple ICC/IF Cell line: HepG2Fixative: 4% Paraformaldehyde Permeabilization: 0.1% Triton X-100 Primary Ab dilution: 1:50 Primary incubation condition: 4°C overnight Secondary Ab: Goat Anti-Rabbit IgG Nuclear counter stain: DAPI (blue) Description: The green color represents the positive signal observed with :
Recombinant Rabbit Monoclonal Anti-Carboxyethyllysine Rabbit mAb Clone Number: 12H4L5 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB ICC/IF Reactivity: All Synonyms: Kcel Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms Kcel UniProt ID / Immunogen Carboxyethylated lysine peptides MW (kDa) Multiple Specificity Anti-Carboxyethyllysine Rabbit mAb detects proteins post-translationally modified by carboxyethylation on lysine residues. This pan antibody recognizes carboxyethylated lysine independent of its surrounding sequences. Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 All ICC/IF 1:50 Human Properties Purity Protein A and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background N epsilon-(carboxyethyl)lysine (CEL), one of the advanced glycation end products (AGEs), is a stable, nonenzymatic chemical modifications of protein lysine residues resulting from glycation and oxidation. Recently, it has been demonstrated that gut microbiota mediates an aging-associated decline in gut barrier function, allowing AGEs to leak into the bloodstream from the gut and impairing microglial function in the brain. CEL was also detected in human lens proteins at a concentration similar to that of CML(CEL homolog), and increased with age in parallel with the concentration of CML. Although CEL was formed in highest yields during the reaction of methylglyoxal and triose phosphates with lysine and protein, it was also formed in reactions of pentoses, ascorbate and other sugars with lysine and RNase. Cellular location Nucleus, Cytoplasm Images Dot Blot Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Peptide amount: 1: 4ng, 20 ng, 100 ng; 2-10: 100 ng Exposure time: 30 secondsThe list of peptides is included in the table below. WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: (-): HepG2; (+): HepG2 + methylglyoxal (2 mM, 24 hours) + trichostatin A (1 uM, 5 hr)Protein loading amount: 20 μgExposure time: 60 sPredicted band size: MultipleObserved band size: Multiple Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: (-): Rat brain; (+): Rat brain was ground +UV (6mJ/cm2, 2 h), followed by protein extractionProtein loading amount: 20 μgExposure time: 60 sPredicted band size: MultipleObserved band size: Multiple Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: (-): Mouse liver; (+): Mouse liver was ground + UV (6mJ/cm2, 2 h), followed by protein extractionProtein loading amount: 20 μgExposure time: 60 sPredicted band size: MultipleObserved band size: Multiple Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: (-): 1: HeLa, 2: SH-SY5Y, 3: BRL, 4:Neuro2aProtein loading amount: 20 μgExposure time: 60 sPredicted band size: MultipleObserved band size: Multiple Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: Rat liver, 2: Mouse brainProtein loading amount: 20 μgExposure time: 30 sPredicted band size: MultipleObserved band size: Multiple ICC/IF Cell line: HepG2Fixative: 4% Paraformaldehyde Permeabilization: 0.1% Triton X-100 Primary Ab dilution: 1:50 Primary incubation condition: 4°C overnight Secondary Ab: Goat Anti-Rabbit IgG Nuclear counter stain: DAPI (blue) Description: The green color represents the positive signal observed with