anti-calretinin rabbit pab

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Anti-Calretinin Rabbit pAb Clone Number: / Host: Rabbit Clonality: Polyclonal Applications： WB IHC-P Reactivity: Human, Mouse, Rat Synonyms: CR, calbindin, CALB2, CAB29 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms CR, calbindin, CALB2, CAB29 UniProt ID P22676 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Mouse, Rat IHC-P 1:500 – 1:2000 Human Properties Purity Protein A and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Calretinin, also named as 29 kDa calbindin and calbindin 2, is a calcium-binding protein of the EF-hand family encoded by the CALB2 gene. It is differentially expressed from homologous family member calbindin-d28k in distinct neuronal populations of the retina, auditory system, and cerebellar granule cells, and acts as a marker for specific neuronal subsets of the subthalamic nucleus and the substantia nigra. Calretinin has been shown to play an important role in modulating neuronal excitability and the induction of long-term potentiation. Research has shown that, pathologically, Calretinin is a selective marker for epithelial mesothelioma, making it a diagnostic tool to differentiate from adenocarcinomas. Cellular location cytosol, dendrite, neuron projection, nucleus, synapse Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgGH&L (HRP Conjugate) Lysate: 1: SH-SY5Y; 2: Mouse brain; 3: Rat brain Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 29 kDa Observed band size: 24 kDa IHC-P Tissue: Human colon carcinoma Section type: Formalin-fixed & Paraffin-embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary Ab dilution: 1:2000 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use) Counter stain: Hematoxylin (Blue) Description: The brown color represents the positive signal observed with :
Anti-Calretinin Rabbit pAb Clone Number: / Host: Rabbit Clonality: Polyclonal Applications： WB IHC-P Reactivity: Human, Mouse, Rat Synonyms: CR, calbindin, CALB2, CAB29 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms CR, calbindin, CALB2, CAB29 UniProt ID P22676 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Mouse, Rat IHC-P 1:500 – 1:2000 Human Properties Purity Protein A and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Calretinin, also named as 29 kDa calbindin and calbindin 2, is a calcium-binding protein of the EF-hand family encoded by the CALB2 gene. It is differentially expressed from homologous family member calbindin-d28k in distinct neuronal populations of the retina, auditory system, and cerebellar granule cells, and acts as a marker for specific neuronal subsets of the subthalamic nucleus and the substantia nigra. Calretinin has been shown to play an important role in modulating neuronal excitability and the induction of long-term potentiation. Research has shown that, pathologically, Calretinin is a selective marker for epithelial mesothelioma, making it a diagnostic tool to differentiate from adenocarcinomas. Cellular location cytosol, dendrite, neuron projection, nucleus, synapse Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgGH&L (HRP Conjugate) Lysate: 1: SH-SY5Y; 2: Mouse brain; 3: Rat brain Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 29 kDa Observed band size: 24 kDa IHC-P Tissue: Human colon carcinoma Section type: Formalin-fixed & Paraffin-embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary Ab dilution: 1:2000 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use) Counter stain: Hematoxylin (Blue) Description: The brown color represents the positive signal observed with