anti-acetyl-coa carboxylase 1 mouse mab

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Recombinant Anti-Acetyl-CoA Carboxylase 1 Mouse mAb Clone Number: JMMR-1662 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB IHC-P Reactivity: Human, Mouse, Rat Synonyms: ACACA, ACAC, ACC1, ACCA Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG2a/kappa Conjugate Unconjugated Synonyms ACACA, ACAC, ACC1, ACCA UniProt ID Q13085 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human, Mouse, Rat IHC-P 1:50 – 1:100 Human, Mouse, Rat Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Acetyl-CoA carboxylase 1(ACC1) belongs to the biotin containing enzyme family. It catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, which is an intermediate substrate playing a pivotal role in the regulation of fatty acid metabolism and energy production. It is the key enzyme in the biosynthesis and oxidation of fatty acids. Phosphorylation by AMPK at Ser79 or by PKA at Ser1200 inhibits the enzymatic activity of ACC. ACC is a potential target of anti-obesity drugs. Cellular location Cytosol Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Mouse IgG H&L pAb(HRP Conjugate)Lysate: 1: SH-SY5Y, 2: HEK-293T, 3: NIH/3T3, 4: PC-12Protein loading quantity: 20 μgExposure time: 60 sPredicted band size: 267 kDaObserved band size: 267 kDa IHC-P Tissue: Human breast cancerSection type: Formalin-fixed & Paraffin – embedded sectionRetrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0Primary Ab dilution: 1:100Primary Ab incubation condition: 1 hour at room temperatureSecondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)Counter stain: Hematoxylin (Blue)Description: The brown color represents the positive signal observed with :
Recombinant Anti-Acetyl-CoA Carboxylase 1 Mouse mAb Clone Number: JMMR-1662 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB IHC-P Reactivity: Human, Mouse, Rat Synonyms: ACACA, ACAC, ACC1, ACCA Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG2a/kappa Conjugate Unconjugated Synonyms ACACA, ACAC, ACC1, ACCA UniProt ID Q13085 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human, Mouse, Rat IHC-P 1:50 – 1:100 Human, Mouse, Rat Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Acetyl-CoA carboxylase 1(ACC1) belongs to the biotin containing enzyme family. It catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, which is an intermediate substrate playing a pivotal role in the regulation of fatty acid metabolism and energy production. It is the key enzyme in the biosynthesis and oxidation of fatty acids. Phosphorylation by AMPK at Ser79 or by PKA at Ser1200 inhibits the enzymatic activity of ACC. ACC is a potential target of anti-obesity drugs. Cellular location Cytosol Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Mouse IgG H&L pAb(HRP Conjugate)Lysate: 1: SH-SY5Y, 2: HEK-293T, 3: NIH/3T3, 4: PC-12Protein loading quantity: 20 μgExposure time: 60 sPredicted band size: 267 kDaObserved band size: 267 kDa IHC-P Tissue: Human breast cancerSection type: Formalin-fixed & Paraffin – embedded sectionRetrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0Primary Ab dilution: 1:100Primary Ab incubation condition: 1 hour at room temperatureSecondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)Counter stain: Hematoxylin (Blue)Description: The brown color represents the positive signal observed with