anti-c-iap2 rabbit mab

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Recombinant Rabbit Monoclonal Anti-c-IAP2 Rabbit mAb Clone Number: JMMR-3039 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB ICC/IF Reactivity: Human Synonyms: hIAP-1, BIRC3, API2, MIHC, RNF49, CIAP2 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG/kappa Conjugate Unconjugated Synonyms hIAP-1, BIRC3, API2, MIHC, RNF49, CIAP2 UniProt ID Q13489 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human ICC/IF 1:50 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background CIAP2 belongs to the inhibitor of apoptosis protein (IAP) family, which consists of an evolutionarily conserved group of apoptosis inhibitors containing a conserved 70 amino acid BIR (baculovirus inhibitor repeat) domain. Overexpression of IAP family members, particularly survivin and livin, in cancer cell lines and primary tumors suggests an important role for these proteins in cancer progression. In general, the IAP proteins function through direct interactions to inhibit the activity of several caspases, including caspase-3, caspase-7, and caspase-9. In addition, binding of IAP family members to the mitochondrial protein Smac blocks their interaction with caspase-9, thereby allowing the processing and activation of the caspase. Cellular location Cytoplasm Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgGH&L(HRP Conjugate) Lysate: 1: Daudi, 2: Raji Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 68 kDa Observed band size: 72 kDa ICC/IF Cell line: Raji Fixative: 100% Ice-cold methanol Permeabilization: 0.1% Triton X-100 Primary Ab dilution: 1:50 Primary Ab incubation condition: 4°C overnight Secondary Ab: Goat Anti-Rabbit IgGNuclear counter stain: DAPI (Blue) Description: The green color represents the positive signal observed with :
Recombinant Rabbit Monoclonal Anti-c-IAP2 Rabbit mAb Clone Number: JMMR-3039 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB ICC/IF Reactivity: Human Synonyms: hIAP-1, BIRC3, API2, MIHC, RNF49, CIAP2 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG/kappa Conjugate Unconjugated Synonyms hIAP-1, BIRC3, API2, MIHC, RNF49, CIAP2 UniProt ID Q13489 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human ICC/IF 1:50 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background CIAP2 belongs to the inhibitor of apoptosis protein (IAP) family, which consists of an evolutionarily conserved group of apoptosis inhibitors containing a conserved 70 amino acid BIR (baculovirus inhibitor repeat) domain. Overexpression of IAP family members, particularly survivin and livin, in cancer cell lines and primary tumors suggests an important role for these proteins in cancer progression. In general, the IAP proteins function through direct interactions to inhibit the activity of several caspases, including caspase-3, caspase-7, and caspase-9. In addition, binding of IAP family members to the mitochondrial protein Smac blocks their interaction with caspase-9, thereby allowing the processing and activation of the caspase. Cellular location Cytoplasm Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgGH&L(HRP Conjugate) Lysate: 1: Daudi, 2: Raji Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 68 kDa Observed band size: 72 kDa ICC/IF Cell line: Raji Fixative: 100% Ice-cold methanol Permeabilization: 0.1% Triton X-100 Primary Ab dilution: 1:50 Primary Ab incubation condition: 4°C overnight Secondary Ab: Goat Anti-Rabbit IgGNuclear counter stain: DAPI (Blue) Description: The green color represents the positive signal observed with