Product Name :
anti-c-cbl mouse mab
Isotype :
IgG1/kappa
Conjugate :
Unconjugated
Synonyms:
RING finger protein 55, CBL, CBL2, RNF55
UniProt ID :
P22681
Immunogen:
MW (kDa) :
Specificity:
Purity :
Protein A purified
Purity :
PBS, Glycerol, BSA
Storage :
Store at -20°C. Avoid freeze/thaw cycles.
Stability:
Stable for 12 months from date of receipt/reconstitution.
Background :
c-Cbl is a ubiquitously expressed cytoplasmic adaptor protein that is especially predominant in hematopoietic cells. c-Cbl is rapidly tyrosine-phosphorylated in response to stimulation of a variety of cell-surface receptors. c-Cbl possesses a highly conserved amino-terminal phosphotyrosine binding domain (TKB) and a C3HC4 RING finger motif. The TKB recognizes phosphorylated tyrosine on activated receptor tyrosine kinases (RTKs) as well as other nonreceptor tyrosine kinases. The RING finger motif recruits ubiquitin-conjugating enzymes. These two domains are primarily responsible for the ubiquitin ligase activity of c-Cbl and downregulation of RTKs. In human cancer tissues, c-Cbl is frequently tyrosine-phosphorylated in a tumor-specific manner. Phosphorylation of Tyr731 of c-Cbl provides a docking site for downstream signaling components such as p85 and Fyn. Cellular location Golgi apparatus, Cytosol, Nucleus
Images :
WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hoursat room temperature Secondary Ab: Goat Anti-Mouse IgGH&L(HRP Conjugate) Lysate: 1: Ramos, 2: RAW264.7 Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 100 kDa Observed band size: 120 kDa ICC/IF Cell line: Ramos Fixative: 4% Paraformaldehyde Permeabilization: 0.1% Triton X-100 Primary Ab dilution: 1:50 Primary Ab incubation condition: 4°C overnight Secondary Ab: Goat Anti-Mouse IgGNuclear counter stain: DAPI (Blue) Description: The green color represents the positive signal observed with
Vapor Pressure :
Recombinant Anti-c-Cbl Mouse mAb Clone Number: JMMR-1393 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB ICC/IF Reactivity: Human, Mouse Synonyms: RING finger protein 55, CBL, CBL2, RNF55 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG1/kappa Conjugate Unconjugated Synonyms RING finger protein 55, CBL, CBL2, RNF55 UniProt ID P22681 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human, Mouse ICC/IF 1:50 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background c-Cbl is a ubiquitously expressed cytoplasmic adaptor protein that is especially predominant in hematopoietic cells. c-Cbl is rapidly tyrosine-phosphorylated in response to stimulation of a variety of cell-surface receptors. c-Cbl possesses a highly conserved amino-terminal phosphotyrosine binding domain (TKB) and a C3HC4 RING finger motif. The TKB recognizes phosphorylated tyrosine on activated receptor tyrosine kinases (RTKs) as well as other nonreceptor tyrosine kinases. The RING finger motif recruits ubiquitin-conjugating enzymes. These two domains are primarily responsible for the ubiquitin ligase activity of c-Cbl and downregulation of RTKs. In human cancer tissues, c-Cbl is frequently tyrosine-phosphorylated in a tumor-specific manner. Phosphorylation of Tyr731 of c-Cbl provides a docking site for downstream signaling components such as p85 and Fyn. Cellular location Golgi apparatus, Cytosol, Nucleus Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hoursat room temperature Secondary Ab: Goat Anti-Mouse IgGH&L(HRP Conjugate) Lysate: 1: Ramos, 2: RAW264.7 Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 100 kDa Observed band size: 120 kDa ICC/IF Cell line: Ramos Fixative: 4% Paraformaldehyde Permeabilization: 0.1% Triton X-100 Primary Ab dilution: 1:50 Primary Ab incubation condition: 4°C overnight Secondary Ab: Goat Anti-Mouse IgGNuclear counter stain: DAPI (Blue) Description: The green color represents the positive signal observed with
Recombinant Anti-c-Cbl Mouse mAb Clone Number: JMMR-1393 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB ICC/IF Reactivity: Human, Mouse Synonyms: RING finger protein 55, CBL, CBL2, RNF55 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG1/kappa Conjugate Unconjugated Synonyms RING finger protein 55, CBL, CBL2, RNF55 UniProt ID P22681 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human, Mouse ICC/IF 1:50 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background c-Cbl is a ubiquitously expressed cytoplasmic adaptor protein that is especially predominant in hematopoietic cells. c-Cbl is rapidly tyrosine-phosphorylated in response to stimulation of a variety of cell-surface receptors. c-Cbl possesses a highly conserved amino-terminal phosphotyrosine binding domain (TKB) and a C3HC4 RING finger motif. The TKB recognizes phosphorylated tyrosine on activated receptor tyrosine kinases (RTKs) as well as other nonreceptor tyrosine kinases. The RING finger motif recruits ubiquitin-conjugating enzymes. These two domains are primarily responsible for the ubiquitin ligase activity of c-Cbl and downregulation of RTKs. In human cancer tissues, c-Cbl is frequently tyrosine-phosphorylated in a tumor-specific manner. Phosphorylation of Tyr731 of c-Cbl provides a docking site for downstream signaling components such as p85 and Fyn. Cellular location Golgi apparatus, Cytosol, Nucleus Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hoursat room temperature Secondary Ab: Goat Anti-Mouse IgGH&L(HRP Conjugate) Lysate: 1: Ramos, 2: RAW264.7 Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 100 kDa Observed band size: 120 kDa ICC/IF Cell line: Ramos Fixative: 4% Paraformaldehyde Permeabilization: 0.1% Triton X-100 Primary Ab dilution: 1:50 Primary Ab incubation condition: 4°C overnight Secondary Ab: Goat Anti-Mouse IgGNuclear counter stain: DAPI (Blue) Description: The green color represents the positive signal observed with
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