Product Name :
anti-butyryllysine rabbit mab
Isotype :
IgG
Conjugate :
Unconjugated
Synonyms:
Kbu
UniProt ID :
/
Immunogen:
Butyrylated lysine peptides
MW (kDa) :
Multiple
Specificity:
Anti-Butyryllysine Rabbit mAb detects proteins post-translationally modified by butyrylation on lysine residues. This pan antibody recognizes butyrylated lysine independent of its surrounding sequences.
Purity :
Protein A purified
Purity :
PBS, Glycerol, BSA
Storage :
Store at -20°C. Avoid freeze/thaw cycles.
Stability:
Stable for 12 months from date of receipt/reconstitution.
Background :
Butyrylation of lysine, structurally similar to lysine acetylation and lysine propionylation, is a newly identified reversible modification controlling protein activity. With integrated proteomic approaches and biochemistry analysis, lysine butyrylation has been well demonstrated in both prokaryotes and eukaryotes in wide ranges of proteins including histones and non-histone substrates. Given the facts that many lysine residues in histones and non-histone substrates, such as p53, p300/CBP, are butyrylated, lysine butyrylation may play a vital role in epigenetic modulation by impacting on chromatin dynamics and plasticity, DNA transcriptional regulation and tumorigenesis, etc. Cellular location /
Images :
Dot Blot Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Peptide amount: 4 ng, 20 ng, 100 ng Exposure time: 30 secondsThe list of peptides is included in the table below. WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: : (-) HeLa; (+) HeLa + sodium butyrate (30 mM, 4 hours)Protein loading amount: 20 μgExposure time: 60 sPredicted band size: MultipleObserved band size: Multiple Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: HeLa, 2: NIH-3T3, 3: BRL, 4: Mouse liver, 5: Mouse kidneyProtein loading amount: 20 μgExposure time: 60 sPredicted band size: MultipleObserved band size: Multiple IHC-P Tissue: Human colon cancer Section type: Formalin fixed & paraffin-embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:500 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use) Counter stain: Hematoxylin (blue) Description: The brown color represents the positive signal observed with
Vapor Pressure :
Recombinant Rabbit Monoclonal Anti-Butyryllysine Rabbit mAb Clone Number: PS
Recombinant Rabbit Monoclonal Anti-Butyryllysine Rabbit mAb Clone Number: PS
Related websites: https://www.medchemexpress.com/antibodies.html
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