anti-butyryllysine mouse mab

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Anti-Butyryllysine Mouse mAb Clone Number: / Host: Mouse Clonality: Monoclonal Applications： WB ICC/IF Reactivity: All Synonyms: Kbu Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms Kbu UniProt ID / Immunogen Butyrylated lysine peptides MW (kDa) Multiple Specificity Anti-Butyryllysine Mouse mAb detects proteins with Butyrylated lysine residues. This pan antibody recognizes Butyrylated lysine independent of its surrounding sequences. Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 All ICC/IF 1:100 Human Properties Purity Protein G and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Butyrylation of lysine, structurally similar to lysine acetylation and lysine propionylation, is a newly identified reversible modification controlling protein activity. With integrated proteomic approaches and biochemistry analysis, lysine butyrylation has been well demonstrated in both prokaryotes and eukaryotes in wide ranges of proteins including histones and non-histone substrates. Given the facts that many lysine residues in histones and non-histone substrates, such as p53, p300/CBP, are butyrylated, lysine butyrylation may play a vital role in epigenetic modulation by impacting on chromatin dynamics and plasticity, DNA transcriptional regulation and tumorigenesis, etc. Cellular location / Images Dot Blot Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)Immunogen peptide quantity: 100 ngExposure time: 60 sThe list of peptides is included in the table below. WB Blocking buffer: 5% NFDM/TBSTPrimary ab dilution: 1:2000Primary ab incubation condition: 2 hours at room temperatureSecondary ab: Goat Anti-Mouse IgG H&L (HRP)Lysate: HeLa, N2a, BRL, Mouse liverProtein loading quantity: 20 μgExposure time: 60 sPredicted MW: Multiple kDaObserved MW: Multiple kDa ICC/IF Cell line: (A) HeLa, (B) HeLa + Sodium butyrate(5mM, 24hr)Fixation: 100% Ice-cold methanolPermeabilization: 0.1% TritonX-100Primary Ab dilution: 1:100Primary Ab incubation condition: 4℃ overnightSecondary Ab: Goat Anti-Rabbit IgGNuclear counter stain: DAPI (blue)Counter stain: Tubulin (Red)Comment: Color green is the positive signal for :
Anti-Butyryllysine Mouse mAb Clone Number: / Host: Mouse Clonality: Monoclonal Applications： WB ICC/IF Reactivity: All Synonyms: Kbu Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms Kbu UniProt ID / Immunogen Butyrylated lysine peptides MW (kDa) Multiple Specificity Anti-Butyryllysine Mouse mAb detects proteins with Butyrylated lysine residues. This pan antibody recognizes Butyrylated lysine independent of its surrounding sequences. Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 All ICC/IF 1:100 Human Properties Purity Protein G and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Butyrylation of lysine, structurally similar to lysine acetylation and lysine propionylation, is a newly identified reversible modification controlling protein activity. With integrated proteomic approaches and biochemistry analysis, lysine butyrylation has been well demonstrated in both prokaryotes and eukaryotes in wide ranges of proteins including histones and non-histone substrates. Given the facts that many lysine residues in histones and non-histone substrates, such as p53, p300/CBP, are butyrylated, lysine butyrylation may play a vital role in epigenetic modulation by impacting on chromatin dynamics and plasticity, DNA transcriptional regulation and tumorigenesis, etc. Cellular location / Images Dot Blot Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)Immunogen peptide quantity: 100 ngExposure time: 60 sThe list of peptides is included in the table below. WB Blocking buffer: 5% NFDM/TBSTPrimary ab dilution: 1:2000Primary ab incubation condition: 2 hours at room temperatureSecondary ab: Goat Anti-Mouse IgG H&L (HRP)Lysate: HeLa, N2a, BRL, Mouse liverProtein loading quantity: 20 μgExposure time: 60 sPredicted MW: Multiple kDaObserved MW: Multiple kDa ICC/IF Cell line: (A) HeLa, (B) HeLa + Sodium butyrate(5mM, 24hr)Fixation: 100% Ice-cold methanolPermeabilization: 0.1% TritonX-100Primary Ab dilution: 1:100Primary Ab incubation condition: 4℃ overnightSecondary Ab: Goat Anti-Rabbit IgGNuclear counter stain: DAPI (blue)Counter stain: Tubulin (Red)Comment: Color green is the positive signal for