anti-butyryl-histone h4 (lys5) mouse mab

Vapor Pressure :
Anti-Butyryl-Histone H4 (Lys5) Mouse mAb Clone Number: / Host: Mouse Clonality: Monoclonal Applications： WB IHC-P ICC/IF ChIP Reactivity: Human, Mouse, Rat Synonyms: H4K5bu Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H4K5bu UniProt ID P62805 Immunogen MW (kDa) 11 Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500-1:2000 Human, Mouse, Rat IHC-P 1:200-1:1000 Human, Mouse, Rat ICC/IF 1:50 Human ChIP 6 μg/5×106 cells Human Properties Purity Protein G and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histones are subject to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, etc. Butyrylation of lysine, structurally similar to lysine acetylation, is a newly identified reversible modification controlling protein activity. The reversible lysine butyrylation has been well demonstrated in both prokaryotes and eukaryotes in wide ranges of proteins including histones and non-histone substrates, such as p53. Similar to acetylation of histione H4 at lysine 12, butyrylation of histone H4 at lysine 5 may play a vital role in the epigenetic modulation, including chromatin remodeling and DNA transcriptional regulation. Cellular location Nucleus Images Dot Blot Blocking buffer: 5% NFDM/TBSTPrimary ab dilution: 1:1000Primary ab incubation condition: 2 hours at room temperatureSecondary ab: Goat Anti-Mouse IgG H&L (HRP)Immunogen peptide quantity: 1 ng, 4 ng, 16 ngExposure time: 60 sThe list of peptides is included in the table below: WB Blocking buffer: 5% NFDM/TBSTPrimary ab dilution: 1:2000Primary ab incubation condition: 2 hours at room temperatureSecondary ab: Goat Anti-Mouse IgG H&L (HRP)Lysate: (-) HeLa, (+) HeLa+Sodium butyrate (30mM, 4 h)Protein loading quantity: 20 μgExposure time: 60 sPredicted MW: 11 kDaObserved MW: 11 kDa IHC-P Tissue: Human spleenSection type: Formalin fixed & Paraffin – embedded sectionRetrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0Primary ab dilution: 1:200Primary ab incubation condition: 1 hour at room temperatureSecondary ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use)Counter stain: Hematoxylin (Blue)Comment: Color brown is the positive signal for :
Anti-Butyryl-Histone H4 (Lys5) Mouse mAb Clone Number: / Host: Mouse Clonality: Monoclonal Applications： WB IHC-P ICC/IF ChIP Reactivity: Human, Mouse, Rat Synonyms: H4K5bu Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H4K5bu UniProt ID P62805 Immunogen MW (kDa) 11 Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500-1:2000 Human, Mouse, Rat IHC-P 1:200-1:1000 Human, Mouse, Rat ICC/IF 1:50 Human ChIP 6 μg/5×106 cells Human Properties Purity Protein G and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histones are subject to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, etc. Butyrylation of lysine, structurally similar to lysine acetylation, is a newly identified reversible modification controlling protein activity. The reversible lysine butyrylation has been well demonstrated in both prokaryotes and eukaryotes in wide ranges of proteins including histones and non-histone substrates, such as p53. Similar to acetylation of histione H4 at lysine 12, butyrylation of histone H4 at lysine 5 may play a vital role in the epigenetic modulation, including chromatin remodeling and DNA transcriptional regulation. Cellular location Nucleus Images Dot Blot Blocking buffer: 5% NFDM/TBSTPrimary ab dilution: 1:1000Primary ab incubation condition: 2 hours at room temperatureSecondary ab: Goat Anti-Mouse IgG H&L (HRP)Immunogen peptide quantity: 1 ng, 4 ng, 16 ngExposure time: 60 sThe list of peptides is included in the table below: WB Blocking buffer: 5% NFDM/TBSTPrimary ab dilution: 1:2000Primary ab incubation condition: 2 hours at room temperatureSecondary ab: Goat Anti-Mouse IgG H&L (HRP)Lysate: (-) HeLa, (+) HeLa+Sodium butyrate (30mM, 4 h)Protein loading quantity: 20 μgExposure time: 60 sPredicted MW: 11 kDaObserved MW: 11 kDa IHC-P Tissue: Human spleenSection type: Formalin fixed & Paraffin – embedded sectionRetrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0Primary ab dilution: 1:200Primary ab incubation condition: 1 hour at room temperatureSecondary ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use)Counter stain: Hematoxylin (Blue)Comment: Color brown is the positive signal for