anti-butyryl-histone h3 (lys23) mouse mab

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Anti-Butyryl-Histone H3 (Lys23) Mouse mAb Clone Number: / Host: Mouse Clonality: Monoclonal Applications： WB IHC-P Reactivity: Human, Mouse, Rat Synonyms: H3K23bu Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H3K23bu UniProt ID P68431 Immunogen Butyrylated human histone H3 (Lys23) peptide MW (kDa) 15 Specificity Anti-Butyryl-Histone H3 (Lys23) Mouse mAb detects histone H3 only when it is butyrylated at Lys23. Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Mouse, Rat IHC-P 1:50 – 1:200 Human, Mouse Properties Purity Protein G and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histones are subject to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, etc. Butyrylation of lysine is a newly identified reversible modification controlling chromosome structure and gene transcription. Lysine butyrylation is highly conserved in eukaryotic cells from worm to human. The unique structure and genomic localization of histone lysine butyrylation suggest that it is mechanistically and functionally different from histone lysine acetylation. Specifically, in both human somatic and rabbit male germ cell genomes, histone butyrylation marks either active promoters or potential enhancers. Butyrylation of histone H3 at Lys18 may play a vital role in the epigenetic modulation, including chromatin remodeling and DNA transcriptional regulation. Cellular location Nucleus Images Dot Blot Peptide amount: 1 ng, 4 ng, 16 ngBlocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:10000 Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate) Exposure time: 60 seconds The list of peptides used in the experiment is provided below.Lane 1: H3K23 butyryl. Lane 2: H3K18 acetyl. Lane 3: H3K18 propionyl. Lane 4: H3K18 butyryl. Lane 5: H3K18 crotonyl. Lane 6: H3K18 Monomethyl.Lane 7: H3K23 acetyl. Lane 8: H3K23 propionyl.Lane 9: H3K23 crotonyl. Lane 10: H3K23 Monomethyl.Lane 11: H3K27 acetyl. Lane 12: H3K27 crotonyl.Lane 13: H3K27 Monomethyl. Lane 14: H3K27 Dimethyl.Lane 15: H3K27 Trimethyl. Lane 16: H3K23 unmodified. WB Lysates: (-): HeLa cells; (+): HeLa cells + sodium butyrate (30 mM, 4 hours) Protein loading amount: 20 μg Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate) Exposure time: 60 seconds Predicted band size: 15 kDa Observed band size: 15 kDa IHC-P Tissue: Human spleenSection type: Formalin fixed & paraffin-embedded sectionRetrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0Primary Ab dilution: 1:200Primary Ab incubation condition: 1 hour at room temperatureSecondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)Counter stain: Hematoxylin (blue)Description: The brown color represents the positive signal observed with :
Anti-Butyryl-Histone H3 (Lys23) Mouse mAb Clone Number: / Host: Mouse Clonality: Monoclonal Applications： WB IHC-P Reactivity: Human, Mouse, Rat Synonyms: H3K23bu Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H3K23bu UniProt ID P68431 Immunogen Butyrylated human histone H3 (Lys23) peptide MW (kDa) 15 Specificity Anti-Butyryl-Histone H3 (Lys23) Mouse mAb detects histone H3 only when it is butyrylated at Lys23. Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Mouse, Rat IHC-P 1:50 – 1:200 Human, Mouse Properties Purity Protein G and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histones are subject to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, etc. Butyrylation of lysine is a newly identified reversible modification controlling chromosome structure and gene transcription. Lysine butyrylation is highly conserved in eukaryotic cells from worm to human. The unique structure and genomic localization of histone lysine butyrylation suggest that it is mechanistically and functionally different from histone lysine acetylation. Specifically, in both human somatic and rabbit male germ cell genomes, histone butyrylation marks either active promoters or potential enhancers. Butyrylation of histone H3 at Lys18 may play a vital role in the epigenetic modulation, including chromatin remodeling and DNA transcriptional regulation. Cellular location Nucleus Images Dot Blot Peptide amount: 1 ng, 4 ng, 16 ngBlocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:10000 Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate) Exposure time: 60 seconds The list of peptides used in the experiment is provided below.Lane 1: H3K23 butyryl. Lane 2: H3K18 acetyl. Lane 3: H3K18 propionyl. Lane 4: H3K18 butyryl. Lane 5: H3K18 crotonyl. Lane 6: H3K18 Monomethyl.Lane 7: H3K23 acetyl. Lane 8: H3K23 propionyl.Lane 9: H3K23 crotonyl. Lane 10: H3K23 Monomethyl.Lane 11: H3K27 acetyl. Lane 12: H3K27 crotonyl.Lane 13: H3K27 Monomethyl. Lane 14: H3K27 Dimethyl.Lane 15: H3K27 Trimethyl. Lane 16: H3K23 unmodified. WB Lysates: (-): HeLa cells; (+): HeLa cells + sodium butyrate (30 mM, 4 hours) Protein loading amount: 20 μg Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate) Exposure time: 60 seconds Predicted band size: 15 kDa Observed band size: 15 kDa IHC-P Tissue: Human spleenSection type: Formalin fixed & paraffin-embedded sectionRetrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0Primary Ab dilution: 1:200Primary Ab incubation condition: 1 hour at room temperatureSecondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)Counter stain: Hematoxylin (blue)Description: The brown color represents the positive signal observed with