Product Name :
anti-butyryl-histone h3 (lys18) rabbit pab
Isotype :
IgG
Conjugate :
Unconjugated
Synonyms:
H3K18bu
UniProt ID :
P68431
Immunogen:
Butyrylated human histone H3 (Lys18) peptide
MW (kDa) :
15
Specificity:
Anti-Butyryl-Histone H3 (Lys18) Rabbit pAb detects histone H3 only when it is butyrylated at Lys18.
Purity :
Protein A and immunogen affinity purified
Purity :
PBS, Glycerol, BSA
Storage :
Store at -20°C. Avoid freeze/thaw cycles.
Stability:
Stable for 12 months from date of receipt/reconstitution.
Background :
Histones are subject to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, etc. Butyrylation of lysine is a newly identified reversible modification controlling chromosome structure and gene transcription. Lysine butyrylation is highly conserved in eukaryotic cells from worm to human. The unique structure and genomic localization of histone lysine butyrylation suggest that it is mechanistically and functionally different from histone lysine acetylation. Specifically, in both human somatic and rabbit male germ cell genomes, histone butyrylation marks either active promoters or potential enhancers. Butyrylation of histone H3 at Lys18 may play a vital role in the epigenetic modulation, including chromatin remodeling and DNA transcriptional regulation Cellular location Nucleus
Images :
Dot Blot Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Immunogen peptide quantity: 1 ng, 4 ng, 16 ngExposure time: 60 sThe list of peptides is included in the table below. WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: (-) HeLa, (+) Hela + sodium butyrate (30mM, 4 hours)Protein loading quantity: 20 μgExposure time: 60 sPredicted band size: 17 kDaObserved band size: 17 kDa ICC/IF Cell line: (A) HeLa, (B) HeLa+ SBA (5mM, 24 hours)Fixative: 4% ParaformaldehydePermeabilization: 0.1% Triton X-100Primary Ab dilution: 1:50Primary Ab incubation condition: 4°C overnightSecondary Ab: Goat Anti-Rabbit IgGNuclear counter stain: DAPI (Blue)Counter stain: Tubulin (Red)Description: The green color represents the positive signal observed with
Vapor Pressure :
Anti-Butyryl-Histone H3 (Lys18) Rabbit pAb This item is discontinued. Please see alternative products below. Clone Number: / Host: Rabbit Clonality: Polyclonal Applications： WB ICC/IF Reactivity: Human, Mouse, Rat Synonyms: H3K18bu Product Size 100 μl Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols Alternative Products View More Images（2） $ 420 Recombinant Rabbit Monoclonal Anti-Butyryl-Histone H3 (Lys18) Rabbit mAb Catalog #
Anti-Butyryl-Histone H3 (Lys18) Rabbit pAb This item is discontinued. Please see alternative products below. Clone Number: / Host: Rabbit Clonality: Polyclonal Applications： WB ICC/IF Reactivity: Human, Mouse, Rat Synonyms: H3K18bu Product Size 100 μl Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols Alternative Products View More Images（2） $ 420 Recombinant Rabbit Monoclonal Anti-Butyryl-Histone H3 (Lys18) Rabbit mAb Catalog #
Related websites: https://www.medchemexpress.com/antibodies.html
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