anti-butyryl-histone h3 (lys18) rabbit mab

Vapor Pressure :
Recombinant Rabbit Monoclonal Anti-Butyryl-Histone H3 (Lys18) Rabbit mAb Clone Number: PA-177-23 Host: Rabbit Clonality: Monoclonal Applications： WB Reactivity: Human Synonyms: H3K18bu Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H3K18bu UniProt ID P68431 Immunogen Butyrylated human histone H3 (Lys18) peptide MW (kDa) 15 Specificity Anti-Butyryl-Histone H3 (Lys18) Rabbit mAb detects histone H3 only when it is butyrylated at Lys18. Product Usage Information Applications Dilution Recommended Species WB 1:500-1:2000 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histones are subject to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, etc. Butyrylation of lysine, structurally similar to lysine acetylation and propionylation, is a newly identified reversible modification controlling chromosome structure and gene transcription. With integrated proteomic approaches and biochemistry analysis, lysine butyrylation has been well demonstrated in both prokaryotes and eukaryotes. The unique structure and genomic localization of histone lysine butyrylation suggest that it is mechanistically and functionally different from histone lysine acetylation. Butyrylation of histone H3 at Lys18 may play a vital role in the epigenetic modulation, including chromatin remodeling and DNA transcriptional regulation. Cellular location Nucleus Images Dot Blot Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP) Peptide amount: 1-2: 1ng, 4ng, 16 ng, 64 ng; 3-9: 64 ng Exposure time: 30 s The list of peptides is included in the table below. WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: (-): HeLa; (+): HeLa + sodium butyrate (30 mM, 4 h) Protein loading amount: 20 μg Exposure time: 30 s Predicted MW: 15 kDa Observed MW: 15 kDa :
Recombinant Rabbit Monoclonal Anti-Butyryl-Histone H3 (Lys18) Rabbit mAb Clone Number: PA-177-23 Host: Rabbit Clonality: Monoclonal Applications： WB Reactivity: Human Synonyms: H3K18bu Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H3K18bu UniProt ID P68431 Immunogen Butyrylated human histone H3 (Lys18) peptide MW (kDa) 15 Specificity Anti-Butyryl-Histone H3 (Lys18) Rabbit mAb detects histone H3 only when it is butyrylated at Lys18. Product Usage Information Applications Dilution Recommended Species WB 1:500-1:2000 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histones are subject to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, etc. Butyrylation of lysine, structurally similar to lysine acetylation and propionylation, is a newly identified reversible modification controlling chromosome structure and gene transcription. With integrated proteomic approaches and biochemistry analysis, lysine butyrylation has been well demonstrated in both prokaryotes and eukaryotes. The unique structure and genomic localization of histone lysine butyrylation suggest that it is mechanistically and functionally different from histone lysine acetylation. Butyrylation of histone H3 at Lys18 may play a vital role in the epigenetic modulation, including chromatin remodeling and DNA transcriptional regulation. Cellular location Nucleus Images Dot Blot Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP) Peptide amount: 1-2: 1ng, 4ng, 16 ng, 64 ng; 3-9: 64 ng Exposure time: 30 s The list of peptides is included in the table below. WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: (-): HeLa; (+): HeLa + sodium butyrate (30 mM, 4 h) Protein loading amount: 20 μg Exposure time: 30 s Predicted MW: 15 kDa Observed MW: 15 kDa