anti-butyryl-histone h3 (lys18) mouse mab

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Anti-Butyryl-Histone H3 (Lys18) Mouse mAb Clone Number: / Host: Mouse Clonality: Monoclonal Applications： WB ChIP Reactivity: Human, Mouse, Rat Synonyms: H3K18bu Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H3K18bu UniProt ID P68431 Immunogen Butyrylated human histone H3 (Lys18) peptide MW (kDa) 15 Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Mouse, Rat ChIP 6 μg/5×106 cells Human Properties Purity Protein G and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histones are subjected to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, etc. Butyrylation of lysine is a newly identified reversible modification controlling chromosome structure and gene transcription. Lysine butyrylation is highly conserved in eukaryotic cells from worm to human. The unique structure and genomic localization of histone lysine butyrylation suggest that it is mechanistically and functionally different from histone lysine acetylation. Specifically, in both human somatic and rabbit male germ cell genomes, histone butyrylation marks either active promoters or potential enhancers. Butyrylation of histone H3 at Lys18 may play a vital role in the epigenetic modulation, including chromatin remodeling and DNA transcriptional regulation. Cellular location Nucleus Images Dot Blot Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)Immunogen peptide quantity: 1 ng, 4 ng, 16 ngExposure time: 60 sThe list of peptides is included in the table below. WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)Lysate: (-) HeLa, (+) HeLa+ Sodium butyrate (30mM, 4 hours)+ Trichostatin A (500ng/ml, 4 hours)Protein loading quantity: 20 μgExposure time: 60 sPredicted band size: 17 kDaObserved band size: 17 kDa Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)Lysate: (-) C2C12, (+) C2C12+ Sodium butyrate (30mM, 4 hours)Protein loading quantity: 20 μgExposure time: 60 sPredicted band size: 17 kDaObserved band size: 17 kDa ChIP Cell type: HeLa+ Sodium butyrate (30 mM, 4 hours)+Trichostatin A (500 ng/ml, 4 hours)Cross-linking conditions: No cross-linkingAmount of chromatin per IP: 5×106 cellsAmount of Ab per IP: 6 ugBeads type and amount per IP: 50 μl of Protein A/G MagBeadsDescription: Chromatin immunoprecipitations were performed with 6 μg of normal mouse IgG as a negative control. The immunoprecipitated DNA was quantified by real-time PCR using primers specific for the human GAPDH CDS region, RPL30 Exon 3, LDHA, FOXO3a-promoter, FOXO3a-downstream and RAB20. The data are presented as enrichment of each sample relative to total amount of input chromatin at each amplicon. :
Anti-Butyryl-Histone H3 (Lys18) Mouse mAb Clone Number: / Host: Mouse Clonality: Monoclonal Applications： WB ChIP Reactivity: Human, Mouse, Rat Synonyms: H3K18bu Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H3K18bu UniProt ID P68431 Immunogen Butyrylated human histone H3 (Lys18) peptide MW (kDa) 15 Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Mouse, Rat ChIP 6 μg/5×106 cells Human Properties Purity Protein G and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histones are subjected to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, etc. Butyrylation of lysine is a newly identified reversible modification controlling chromosome structure and gene transcription. Lysine butyrylation is highly conserved in eukaryotic cells from worm to human. The unique structure and genomic localization of histone lysine butyrylation suggest that it is mechanistically and functionally different from histone lysine acetylation. Specifically, in both human somatic and rabbit male germ cell genomes, histone butyrylation marks either active promoters or potential enhancers. Butyrylation of histone H3 at Lys18 may play a vital role in the epigenetic modulation, including chromatin remodeling and DNA transcriptional regulation. Cellular location Nucleus Images Dot Blot Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)Immunogen peptide quantity: 1 ng, 4 ng, 16 ngExposure time: 60 sThe list of peptides is included in the table below. WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)Lysate: (-) HeLa, (+) HeLa+ Sodium butyrate (30mM, 4 hours)+ Trichostatin A (500ng/ml, 4 hours)Protein loading quantity: 20 μgExposure time: 60 sPredicted band size: 17 kDaObserved band size: 17 kDa Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)Lysate: (-) C2C12, (+) C2C12+ Sodium butyrate (30mM, 4 hours)Protein loading quantity: 20 μgExposure time: 60 sPredicted band size: 17 kDaObserved band size: 17 kDa ChIP Cell type: HeLa+ Sodium butyrate (30 mM, 4 hours)+Trichostatin A (500 ng/ml, 4 hours)Cross-linking conditions: No cross-linkingAmount of chromatin per IP: 5×106 cellsAmount of Ab per IP: 6 ugBeads type and amount per IP: 50 μl of Protein A/G MagBeadsDescription: Chromatin immunoprecipitations were performed with 6 μg of normal mouse IgG as a negative control. The immunoprecipitated DNA was quantified by real-time PCR using primers specific for the human GAPDH CDS region, RPL30 Exon 3, LDHA, FOXO3a-promoter, FOXO3a-downstream and RAB20. The data are presented as enrichment of each sample relative to total amount of input chromatin at each amplicon.