anti-butyryl-histone h3 (lys14) rabbit mab

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Recombinant Rabbit Monoclonal Anti-Butyryl-Histone H3 (Lys14) Rabbit mAb Clone Number: PA-039-24BH0L0 Host: Rabbit Clonality: Monoclonal Applications： WB Reactivity: Human, Mouse, Rat Synonyms: H3K14bu Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H3K14bu UniProt ID P68431 Immunogen MW (kDa) 15 Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500-1:2000 Human, Mouse, Rat Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background H3 is core component of nucleosome. Nucleosomes wrap and compact DNAinto chromatin, limiting DNA accessibility to the cellular machineries whichrequire DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Histones are subjected to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, etc. Butyrylation of histones marks active promoters and competes with histone acetylation, which occurs during late Cellular location Nucleus Images Dot Blot Blocking buffer: 5% NFDM/TBSTPrimary ab dilution: 1:2000Primary ab incubation condition: 2 hours at room temperatureSecondary ab: Goat Anti-Rabbit IgG H&L (HRP)Immunogen peptide quantity: 1 ng, 4 ng, 16ng, 64 ngExposure time: 60 sThe list of peptides is included in the table below: WB Blocking buffer: 5% NFDM/TBSTPrimary ab dilution: 1:2000Primary ab incubation condition: 2 hours at room temperatureSecondary ab: Goat Anti-Rabbit IgG H&L(HRP)Lysate: (-): MCF-7+serum starvation (14 h), (+): MCF-7+ sodium butyrate (50 mM, 24 h) +trichostain A (500 ng/ml, 4 h)Protein loading quantity: 20 μgExposure time: 60 sPredicted MW: 15 kDaObserved MW: 15 kDa Blocking buffer: 5% NFDM/TBSTPrimary ab dilution: 1:2000Primary ab incubation condition: 2 hours at room temperatureSecondary ab: Goat Anti-Rabbit IgG H&L(HRP)Lysate: (-): HeLa, (+): HeLa+ sodiumbutyrate(30 mM, 4 h)Protein loading quantity: 20 μgExposure time: 60 sPredicted MW: 15 kDaObserved MW: 15 kDa Blocking buffer: 5% NFDM/TBSTPrimary ab dilution: 1:2000Primary ab incubation condition: 2 hours at room temperatureSecondary ab: Goat Anti-Rabbit IgG H&L(HRP)Lysate: 1: Neuro-2a, 2: BRLProtein loading quantity: 20 μgExposure time: 60 sPredicted MW: 15 kDaObserved MW: 15 kDa :
Recombinant Rabbit Monoclonal Anti-Butyryl-Histone H3 (Lys14) Rabbit mAb Clone Number: PA-039-24BH0L0 Host: Rabbit Clonality: Monoclonal Applications： WB Reactivity: Human, Mouse, Rat Synonyms: H3K14bu Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H3K14bu UniProt ID P68431 Immunogen MW (kDa) 15 Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500-1:2000 Human, Mouse, Rat Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background H3 is core component of nucleosome. Nucleosomes wrap and compact DNAinto chromatin, limiting DNA accessibility to the cellular machineries whichrequire DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Histones are subjected to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, etc. Butyrylation of histones marks active promoters and competes with histone acetylation, which occurs during late Cellular location Nucleus Images Dot Blot Blocking buffer: 5% NFDM/TBSTPrimary ab dilution: 1:2000Primary ab incubation condition: 2 hours at room temperatureSecondary ab: Goat Anti-Rabbit IgG H&L (HRP)Immunogen peptide quantity: 1 ng, 4 ng, 16ng, 64 ngExposure time: 60 sThe list of peptides is included in the table below: WB Blocking buffer: 5% NFDM/TBSTPrimary ab dilution: 1:2000Primary ab incubation condition: 2 hours at room temperatureSecondary ab: Goat Anti-Rabbit IgG H&L(HRP)Lysate: (-): MCF-7+serum starvation (14 h), (+): MCF-7+ sodium butyrate (50 mM, 24 h) +trichostain A (500 ng/ml, 4 h)Protein loading quantity: 20 μgExposure time: 60 sPredicted MW: 15 kDaObserved MW: 15 kDa Blocking buffer: 5% NFDM/TBSTPrimary ab dilution: 1:2000Primary ab incubation condition: 2 hours at room temperatureSecondary ab: Goat Anti-Rabbit IgG H&L(HRP)Lysate: (-): HeLa, (+): HeLa+ sodiumbutyrate(30 mM, 4 h)Protein loading quantity: 20 μgExposure time: 60 sPredicted MW: 15 kDaObserved MW: 15 kDa Blocking buffer: 5% NFDM/TBSTPrimary ab dilution: 1:2000Primary ab incubation condition: 2 hours at room temperatureSecondary ab: Goat Anti-Rabbit IgG H&L(HRP)Lysate: 1: Neuro-2a, 2: BRLProtein loading quantity: 20 μgExposure time: 60 sPredicted MW: 15 kDaObserved MW: 15 kDa