anti-butyryl-histone h2b (lys23) rabbit mab

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Recombinant Rabbit Monoclonal Anti-Butyryl-Histone H2B (Lys23) Rabbit mAb Clone Number: PD-154-27 Host: Rabbit Clonality: Monoclonal Applications： WB Reactivity: Human, Mouse, Rat Synonyms: H2BK23bu Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H2BK23bu UniProt ID P62807 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500-1:2000 Human，Mouse，Rat Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histones are subject to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, etc. Butyrylation of lysine is a newly identified reversible modification controlling chromosome structure and gene transcription. Lysine butyrylation is highly conserved in eukaryotic cells from worm to human. The unique structure and genomic localization of histone lysine butyrylation suggest that it is mechanistically and functionally different from histone lysine acetylation. Specifically, in both human somatic and rabbit male germ cell genomes, histone butyrylation marks either active promoters or potential enhancers. Butyrylation of histone H3 at Lys23 may play a vital role in the epigenetic modulation, including chromatin remodeling and DNA transcriptional regulation. Cellular location Nucleus Images Dot Blot Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L (HRP)Immunogen peptide quantity: 1 ng, 4 ng, 16 ng, 640 ngExposure time: 60 sThe list of peptides is included in the table below: WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L (HRP)Lysate: (-): MCF-7; (+): MCF-7+ Sodiumbutyrate (50mM, 24hr) + Trichostain A (500ng/ml, 4 hr)Protein loading quantity: 20 μgExposure time: 60 sPredicted MW: 14 kDaObserved MW: 14 kDa Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L (HRP)Lysate: (-): HeLa; (+): HeLa+ Sodium butyrate (5mM, 24hr)Protein loading quantity: 20 μgExposure time: 60 sPredicted MW: 14 kDaObserved MW: 14 kDa Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L (HRP)Lysate: (-): NIH/3T3; (+): NIH/3T3 + Trichostain A (500ng/ml, 4 hr)Protein loading quantity: 20 μgExposure time: 60 sPredicted MW: 14 kDaObserved MW: 14 kDa Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L (HRP)Lysate: (-): C6; (+): C6 + Trichostain A (500ng/ml, 4 hr)Protein loading quantity: 20 μgExposure time: 60 sPredicted MW: 14 kDaObserved MW: 14 kDa :
Recombinant Rabbit Monoclonal Anti-Butyryl-Histone H2B (Lys23) Rabbit mAb Clone Number: PD-154-27 Host: Rabbit Clonality: Monoclonal Applications： WB Reactivity: Human, Mouse, Rat Synonyms: H2BK23bu Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H2BK23bu UniProt ID P62807 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500-1:2000 Human，Mouse，Rat Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histones are subject to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, etc. Butyrylation of lysine is a newly identified reversible modification controlling chromosome structure and gene transcription. Lysine butyrylation is highly conserved in eukaryotic cells from worm to human. The unique structure and genomic localization of histone lysine butyrylation suggest that it is mechanistically and functionally different from histone lysine acetylation. Specifically, in both human somatic and rabbit male germ cell genomes, histone butyrylation marks either active promoters or potential enhancers. Butyrylation of histone H3 at Lys23 may play a vital role in the epigenetic modulation, including chromatin remodeling and DNA transcriptional regulation. Cellular location Nucleus Images Dot Blot Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L (HRP)Immunogen peptide quantity: 1 ng, 4 ng, 16 ng, 640 ngExposure time: 60 sThe list of peptides is included in the table below: WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L (HRP)Lysate: (-): MCF-7; (+): MCF-7+ Sodiumbutyrate (50mM, 24hr) + Trichostain A (500ng/ml, 4 hr)Protein loading quantity: 20 μgExposure time: 60 sPredicted MW: 14 kDaObserved MW: 14 kDa Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L (HRP)Lysate: (-): HeLa; (+): HeLa+ Sodium butyrate (5mM, 24hr)Protein loading quantity: 20 μgExposure time: 60 sPredicted MW: 14 kDaObserved MW: 14 kDa Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L (HRP)Lysate: (-): NIH/3T3; (+): NIH/3T3 + Trichostain A (500ng/ml, 4 hr)Protein loading quantity: 20 μgExposure time: 60 sPredicted MW: 14 kDaObserved MW: 14 kDa Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L (HRP)Lysate: (-): C6; (+): C6 + Trichostain A (500ng/ml, 4 hr)Protein loading quantity: 20 μgExposure time: 60 sPredicted MW: 14 kDaObserved MW: 14 kDa