anti-brd8 mouse mab

Vapor Pressure :
Recombinant Anti-BRD8 Mouse mAb Clone Number: 11501 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB IHC-P Reactivity: Human Synonyms: SMAP, SMAP2 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG2a/kappa Conjugate Unconjugated Synonyms SMAP, SMAP2 UniProt ID Q9H0E9 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:200 – 1:1000 Human IHC-P 1:50 – 1:100 Human Properties Purity Protein G purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background May act as a coactivator during transcriptional activation by hormone-activated nuclear receptors (NR). Isoform 2 stimulates transcriptional activation by AR/DHTR, ESR1/NR3A1, RXRA/NR2B1 and THRB/ERBA2. At least isoform 1 and isoform 2 are components of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome – DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. Cellular location Nucleus Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 4°C overnightLysate: 1: HeLa, 2: HeLa nuclearProtein loading quantity: 20 μgExposure time: 30 sPredicted band size: 120 kDaObserved band size: 120 kDa IHC-P Tissue: Human colon cancerSection type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100Primary Ab incubation condition: 1 hour at room temperature Counter stain: HematoxylinDescription: The brown color represents the positive signal observed with :
Recombinant Anti-BRD8 Mouse mAb Clone Number: 11501 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB IHC-P Reactivity: Human Synonyms: SMAP, SMAP2 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG2a/kappa Conjugate Unconjugated Synonyms SMAP, SMAP2 UniProt ID Q9H0E9 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:200 – 1:1000 Human IHC-P 1:50 – 1:100 Human Properties Purity Protein G purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background May act as a coactivator during transcriptional activation by hormone-activated nuclear receptors (NR). Isoform 2 stimulates transcriptional activation by AR/DHTR, ESR1/NR3A1, RXRA/NR2B1 and THRB/ERBA2. At least isoform 1 and isoform 2 are components of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome – DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. Cellular location Nucleus Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 4°C overnightLysate: 1: HeLa, 2: HeLa nuclearProtein loading quantity: 20 μgExposure time: 30 sPredicted band size: 120 kDaObserved band size: 120 kDa IHC-P Tissue: Human colon cancerSection type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100Primary Ab incubation condition: 1 hour at room temperature Counter stain: HematoxylinDescription: The brown color represents the positive signal observed with