anti-beta-amyloid (1-40) rabbit pab

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Anti-beta-Amyloid (1-40) Rabbit pAb Clone Number: / Host: Rabbit Clonality: Polyclonal Applications： WB IHC-P Reactivity: Mouse Synonyms: Beta-APP40 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms Beta-APP40 UniProt ID P05067 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:1000 – 1:5000 Mouse IHC-P 1:500 – 1:2000 Mouse Properties Purity Protein A and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background β-Amyloid (Aβ) precursor protein (APP) is a 100-140 kDa transmembrane glycoprotein that exists as several isoforms The amino acid sequence of APP contains the amyloid domain, which can be released by a two-step proteolytic cleavage. The extracellular deposition and accumulation of the released Aβ fragments form the main components of amyloid plaques in Alzheimer’s disease. APP can be phosphorylated at several sites, which may affect the proteolytic processing and secretion of this protein. Phosphorylation at Thr668 (a position corresponding to the APP695 isoform) by cyclin-dependent kinase is cell-cycle dependent and peaks during G2/M phase. APP phosphorylated at Thr668 exists in adult rat brain and correlates with cultured neuronal differentiation. β -amyloid (1-40) Rabbit pAb recognizes endogenous levels of Aβ-40 peptide. Cellular location Membrane Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:5000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgGH&L (HRP Conjugate)Lysate: 1.Mouse cerebral cortex; 2. APP,PS1mouse cerebral cortex Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 4 kDa Observed band size: 10 kDa IHC-P Tissue: APP,PS1 mouse cerebrumSection type: Formalin-fixed & Paraffin-embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary Ab dilution: 1:2000 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use) Counter stain: Hematoxylin (Blue) Description: The brown color represents the positive signal observed with :
Anti-beta-Amyloid (1-40) Rabbit pAb Clone Number: / Host: Rabbit Clonality: Polyclonal Applications： WB IHC-P Reactivity: Mouse Synonyms: Beta-APP40 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms Beta-APP40 UniProt ID P05067 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:1000 – 1:5000 Mouse IHC-P 1:500 – 1:2000 Mouse Properties Purity Protein A and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background β-Amyloid (Aβ) precursor protein (APP) is a 100-140 kDa transmembrane glycoprotein that exists as several isoforms The amino acid sequence of APP contains the amyloid domain, which can be released by a two-step proteolytic cleavage. The extracellular deposition and accumulation of the released Aβ fragments form the main components of amyloid plaques in Alzheimer’s disease. APP can be phosphorylated at several sites, which may affect the proteolytic processing and secretion of this protein. Phosphorylation at Thr668 (a position corresponding to the APP695 isoform) by cyclin-dependent kinase is cell-cycle dependent and peaks during G2/M phase. APP phosphorylated at Thr668 exists in adult rat brain and correlates with cultured neuronal differentiation. β -amyloid (1-40) Rabbit pAb recognizes endogenous levels of Aβ-40 peptide. Cellular location Membrane Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:5000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgGH&L (HRP Conjugate)Lysate: 1.Mouse cerebral cortex; 2. APP,PS1mouse cerebral cortex Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 4 kDa Observed band size: 10 kDa IHC-P Tissue: APP,PS1 mouse cerebrumSection type: Formalin-fixed & Paraffin-embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary Ab dilution: 1:2000 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use) Counter stain: Hematoxylin (Blue) Description: The brown color represents the positive signal observed with