anti-beta actin mouse mab

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Recombinant Anti-beta Actin Mouse mAb Clone Number: 10178 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB IHC-P ICC/IF Reactivity: Human, Mouse, Rat, A.thaliana, Rice Synonyms: ACTB Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG1 Conjugate Unconjugated Synonyms ACTB UniProt ID P60709 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:1000 – 1:2000 Human, Mouse, Rat, Arabidopsis thaliana, Rice IHC-P 1:50 – 1:100 Human, Mouse ICC/IF 1:50 – 1:100 Human, Mouse Properties Purity Protein G purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells. Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction. In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA. Cellular location Cytoskeleton, Cytoplasm Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureLysate: 1: HeLa, 2: HepG2, 3: Neuro-2a, 4: EL4.IL-2, 5: BRLProtein loading quantity: 20 μgExposure time: 3 sPredicted band size: 41 kDaObserved band size: 45 kDa Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureLysate: 1: Arabidopsis leaves, 2: Rice leavesProtein loading quantity: 20 μgExposure time: 60 sPredicted band size: 41 kDaObserved band size: 45 kDa IHC-P Tissue: Human colonSection type: Formalin-fixed & Paraffin -embedded section Retrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100Primary Ab incubation condition: 1 hour at room temperature Counter stain: HematoxylinDescription: The brown color represents the positive signal observed with :
Recombinant Anti-beta Actin Mouse mAb Clone Number: 10178 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB IHC-P ICC/IF Reactivity: Human, Mouse, Rat, A.thaliana, Rice Synonyms: ACTB Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG1 Conjugate Unconjugated Synonyms ACTB UniProt ID P60709 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:1000 – 1:2000 Human, Mouse, Rat, Arabidopsis thaliana, Rice IHC-P 1:50 – 1:100 Human, Mouse ICC/IF 1:50 – 1:100 Human, Mouse Properties Purity Protein G purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells. Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction. In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA. Cellular location Cytoskeleton, Cytoplasm Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureLysate: 1: HeLa, 2: HepG2, 3: Neuro-2a, 4: EL4.IL-2, 5: BRLProtein loading quantity: 20 μgExposure time: 3 sPredicted band size: 41 kDaObserved band size: 45 kDa Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureLysate: 1: Arabidopsis leaves, 2: Rice leavesProtein loading quantity: 20 μgExposure time: 60 sPredicted band size: 41 kDaObserved band size: 45 kDa IHC-P Tissue: Human colonSection type: Formalin-fixed & Paraffin -embedded section Retrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100Primary Ab incubation condition: 1 hour at room temperature Counter stain: HematoxylinDescription: The brown color represents the positive signal observed with