anti-bcl-2 rabbit mab

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Recombinant Rabbit Monoclonal Anti-Bcl-2 Rabbit mAb Clone Number: 10594 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB ICC/IF Reactivity: Human Synonyms: BCL2, Apoptosis regulator Bcl-2 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG/kappa Conjugate Unconjugated Synonyms BCL2, Apoptosis regulator Bcl-2 UniProt ID P10415 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human ICC/IF 1:50 Human Properties Purity Protein G purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Bcl-2 (B-cell lymphoma 2), encoded in humans by the BCL2 gene, is the founding member of the Bcl-2 family of regulator proteins that regulate cell death (apoptosis), by either inhibiting (anti-apoptotic) or inducing (pro-apoptotic) apoptosis.It was the first apoptosis regulator identified in any organism. Besides, it suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. It regulates cell death by controlling the mitochondrial membrane permeability, and appears to function in a feedback loop system with caspases. It inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis – activating factor (APAF-1). And it may attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release. Cellular location Mitochondrion outer membrane, Nucleus membrane, Endoplasmic reticulum membrane Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: THP-1Protein loading quantity: 20 μgExposure time: 30 sPredicted band size: 26 kDaObserved band size: 26 kDa Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: SH-SY5Y, 2: Jurkat, 3: RajiProtein loading quantity: 20 μgExposure time: 30 sPredicted band size: 26 kDaObserved band size: 26 kDa ICC/IF Cell line: JurkatFixative: 4% ParaformaldehydePermeabilization: 0.1% Triton X-100Primary Ab dilution: 1:50Primary Ab incubation condition: 4°C overnightSecondary Ab: Goat Anti-Mouse IgGNuclear counter stain: DAPI (Blue)Description: The green color represents the positive signal observed with :
Recombinant Rabbit Monoclonal Anti-Bcl-2 Rabbit mAb Clone Number: 10594 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB ICC/IF Reactivity: Human Synonyms: BCL2, Apoptosis regulator Bcl-2 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG/kappa Conjugate Unconjugated Synonyms BCL2, Apoptosis regulator Bcl-2 UniProt ID P10415 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human ICC/IF 1:50 Human Properties Purity Protein G purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Bcl-2 (B-cell lymphoma 2), encoded in humans by the BCL2 gene, is the founding member of the Bcl-2 family of regulator proteins that regulate cell death (apoptosis), by either inhibiting (anti-apoptotic) or inducing (pro-apoptotic) apoptosis.It was the first apoptosis regulator identified in any organism. Besides, it suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. It regulates cell death by controlling the mitochondrial membrane permeability, and appears to function in a feedback loop system with caspases. It inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis – activating factor (APAF-1). And it may attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release. Cellular location Mitochondrion outer membrane, Nucleus membrane, Endoplasmic reticulum membrane Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: THP-1Protein loading quantity: 20 μgExposure time: 30 sPredicted band size: 26 kDaObserved band size: 26 kDa Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: SH-SY5Y, 2: Jurkat, 3: RajiProtein loading quantity: 20 μgExposure time: 30 sPredicted band size: 26 kDaObserved band size: 26 kDa ICC/IF Cell line: JurkatFixative: 4% ParaformaldehydePermeabilization: 0.1% Triton X-100Primary Ab dilution: 1:50Primary Ab incubation condition: 4°C overnightSecondary Ab: Goat Anti-Mouse IgGNuclear counter stain: DAPI (Blue)Description: The green color represents the positive signal observed with