anti-bcl-2 rabbit mab

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Recombinant Rabbit Monoclonal Anti-Bcl-2 Rabbit mAb Clone Number: JRMR-10176-8 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P ICC/IF FC IP Reactivity: Human Synonyms: BCL2, Apoptosis regulator Bcl-2 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms BCL2, Apoptosis regulator Bcl-2 UniProt ID P10415 Immunogen Recombinant human Bcl-2 fragment MW (kDa) 26 Specificity Anti-Bcl-2 Rabbit mAb recognizes endogeneous level of Bcl-2 protein. Product Usage Information Applications Dilution Recommended Species WB 1:1000 – 1:5000 Human IHC-P 1:50 – 1:500 Human ICC/IF 1:50 Human FC 1:100 Human IP 1:50 – 1:100 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release. Cellular location Membrane Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:5000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: Jurkat, 2: MCF-7, 3: THP-1Protein loading quantity: 20 μgExposure time: 60 sPredicted band size: 26 kDaObserved band size: 26 kDa IHC-P Tissue: Human tonsilSection type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:500Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)Counter stain: Hematoxylin (Blue)Description: The brown color represents the positive signal observed with :
Recombinant Rabbit Monoclonal Anti-Bcl-2 Rabbit mAb Clone Number: JRMR-10176-8 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P ICC/IF FC IP Reactivity: Human Synonyms: BCL2, Apoptosis regulator Bcl-2 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms BCL2, Apoptosis regulator Bcl-2 UniProt ID P10415 Immunogen Recombinant human Bcl-2 fragment MW (kDa) 26 Specificity Anti-Bcl-2 Rabbit mAb recognizes endogeneous level of Bcl-2 protein. Product Usage Information Applications Dilution Recommended Species WB 1:1000 – 1:5000 Human IHC-P 1:50 – 1:500 Human ICC/IF 1:50 Human FC 1:100 Human IP 1:50 – 1:100 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release. Cellular location Membrane Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:5000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: Jurkat, 2: MCF-7, 3: THP-1Protein loading quantity: 20 μgExposure time: 60 sPredicted band size: 26 kDaObserved band size: 26 kDa IHC-P Tissue: Human tonsilSection type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:500Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)Counter stain: Hematoxylin (Blue)Description: The brown color represents the positive signal observed with