anti-b7-h4 rabbit mab

Vapor Pressure :
Recombinant Rabbit Monoclonal Anti-B7-H4 Rabbit mAb Clone Number: JRMR-10624-38 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB Reactivity: Human Synonyms: V-set domain-containing T-cell activation inhibitor 1, B7h.5, Protein B7S1, VTCN1 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms V-set domain-containing T-cell activation inhibitor 1, B7h.5, Protein B7S1, VTCN1 UniProt ID Q7Z7D3 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background B7 homolog 4 (B7-H4), also known as VTCN1, is a member of the B7 family of cell surface ligands that regulate T cell activation and immune responses. B7-H4 protein contains two extracellular Ig-like V-type domains, a transmembrane domain, and a short, two amino acid intracellular domain. The B7-H4 protein is shown to inhibit T cell activation, proliferation, and cytokine production. Although B7-H4 mRNA is widely expressed, B7-H4 protein is restricted to antigen presenting cells and B cells. The B7-H4 protein is also found in several tumor types, including ovarian cancer and breast cancer. Research studies indicate that B7-H4 protein is present on the surface of ovarian tumor cells, and that targeted inhibition of B7-H4 using recombinant antibodies restores T cell activation pathways. These studies suggest some potential therapeutic value in blocking B7-H4 function and restoring T cell function in cancer patients. Cellular location Membrane Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgGH&L (HRP Conjugate) Lysate: 1: HT-29; 2: HeLa; 3: T47DProtein loading quantity: 20 μg Exposure time: 30 secondsPredicted band size: 20 / 31 kDa Observed band size: 45 / 65 kDa :
Recombinant Rabbit Monoclonal Anti-B7-H4 Rabbit mAb Clone Number: JRMR-10624-38 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB Reactivity: Human Synonyms: V-set domain-containing T-cell activation inhibitor 1, B7h.5, Protein B7S1, VTCN1 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms V-set domain-containing T-cell activation inhibitor 1, B7h.5, Protein B7S1, VTCN1 UniProt ID Q7Z7D3 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background B7 homolog 4 (B7-H4), also known as VTCN1, is a member of the B7 family of cell surface ligands that regulate T cell activation and immune responses. B7-H4 protein contains two extracellular Ig-like V-type domains, a transmembrane domain, and a short, two amino acid intracellular domain. The B7-H4 protein is shown to inhibit T cell activation, proliferation, and cytokine production. Although B7-H4 mRNA is widely expressed, B7-H4 protein is restricted to antigen presenting cells and B cells. The B7-H4 protein is also found in several tumor types, including ovarian cancer and breast cancer. Research studies indicate that B7-H4 protein is present on the surface of ovarian tumor cells, and that targeted inhibition of B7-H4 using recombinant antibodies restores T cell activation pathways. These studies suggest some potential therapeutic value in blocking B7-H4 function and restoring T cell function in cancer patients. Cellular location Membrane Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgGH&L (HRP Conjugate) Lysate: 1: HT-29; 2: HeLa; 3: T47DProtein loading quantity: 20 μg Exposure time: 30 secondsPredicted band size: 20 / 31 kDa Observed band size: 45 / 65 kDa