anti-5t4 rabbit mab

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Recombinant Rabbit Monoclonal Anti-5T4 Rabbit mAb Clone Number: JRMR- 10716-23- Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P IP Reactivity: Human Synonyms: TPBG, M6P1 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms TPBG, M6P1 UniProt ID Q13641 Immunogen Recombinant human 5T4 fragment MW (kDa) 70-80/85 Specificity Anti-5T4 Rabbit mAb recognizes endogeneous level of 5T4 protein. Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human IHC-P 1:500 – 1:2000 Human IP 1:50 – 1:100 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Trophoblast glycoprotein (encoded by 5T4) is a leucine-rich transmembrane glycoprotein that may be involved in cell adhesion. This protein is an oncofetal antigen that is specific to trophoblast cells. In adults this protein is highly expressed in many tumor cells and is associated with poor clinical outcome in numerous cancers. Cellular location membrane Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: MCF-7, 2: JARProtein loading quantity: 20 μgExposure time: 60 sPredicted band size: 46 kDaObserved band size: 70-80/85 kDa IHC-P Tissue: Human placentaSection type: Formalin-fixed & Paraffin -embedded section Retrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:2000Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)Counter stain: Hematoxylin (Blue)Description: The brown color represents the positive signal observed with :
Recombinant Rabbit Monoclonal Anti-5T4 Rabbit mAb Clone Number: JRMR- 10716-23- Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P IP Reactivity: Human Synonyms: TPBG, M6P1 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms TPBG, M6P1 UniProt ID Q13641 Immunogen Recombinant human 5T4 fragment MW (kDa) 70-80/85 Specificity Anti-5T4 Rabbit mAb recognizes endogeneous level of 5T4 protein. Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human IHC-P 1:500 – 1:2000 Human IP 1:50 – 1:100 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Trophoblast glycoprotein (encoded by 5T4) is a leucine-rich transmembrane glycoprotein that may be involved in cell adhesion. This protein is an oncofetal antigen that is specific to trophoblast cells. In adults this protein is highly expressed in many tumor cells and is associated with poor clinical outcome in numerous cancers. Cellular location membrane Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: MCF-7, 2: JARProtein loading quantity: 20 μgExposure time: 60 sPredicted band size: 46 kDaObserved band size: 70-80/85 kDa IHC-P Tissue: Human placentaSection type: Formalin-fixed & Paraffin -embedded section Retrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:2000Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)Counter stain: Hematoxylin (Blue)Description: The brown color represents the positive signal observed with