anti-asymmetric dimethylarginine rabbit mab

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Recombinant Rabbit Monoclonal Anti-Asymmetric Dimethylarginine Rabbit mAb Clone Number: PA-058-28 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB Reactivity: Human, Mouse, Rat Synonyms: Rme2a Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms Rme2a UniProt ID / Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 All Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histone post-translational modifications (PTMs) are a key mechanism in epigenetic regulation of chromatin structure and are known as the “histone code.” Post-translational modifications on histones include acetylation, methylation, phosphorylation, and some novel acylation modifications discovered in recent years. These histone modifications directly affect the binding of chromatin to transcription factors or other epiregulators, altering genome stability and gene transcription, etc. Histone methylation typically occurs in lysine and arginine residues of core histones. Histone methylation can either promote or inhibit gene transcription, depending on whether histone methylation occurs in lysine or arginine and the number of methylation groups (lysine can be one, two, and trimethylation, arginine can be single, symmetric, and asymmetric dimethylation). Histone lysine methylation usually occurs at histone H3 4,9,27,36,79 and histone H4 20th lysine; Arginine methylation usually occurs at histone H3 at positions 2, 8, 17, 26 and histone H4 at position 3. Protein methylase (HMT) and histone demethylase (HDM) are the main regulators of methylation modification. Cellular location / Images Dot Blot Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Immunogen peptide quantity: 4 ng, 20 ng, 100 ngExposure time: 60 sThe list of peptides is included in the table below. WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: HeLa, 2: SHSY-5Y, 3: NIH/3T3, 4: BRLProtein loading quantity: 20 μgExposure time: 60 sPredicted band size: MultipleObserved band size: Multiple :
Recombinant Rabbit Monoclonal Anti-Asymmetric Dimethylarginine Rabbit mAb Clone Number: PA-058-28 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB Reactivity: Human, Mouse, Rat Synonyms: Rme2a Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms Rme2a UniProt ID / Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 All Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histone post-translational modifications (PTMs) are a key mechanism in epigenetic regulation of chromatin structure and are known as the “histone code.” Post-translational modifications on histones include acetylation, methylation, phosphorylation, and some novel acylation modifications discovered in recent years. These histone modifications directly affect the binding of chromatin to transcription factors or other epiregulators, altering genome stability and gene transcription, etc. Histone methylation typically occurs in lysine and arginine residues of core histones. Histone methylation can either promote or inhibit gene transcription, depending on whether histone methylation occurs in lysine or arginine and the number of methylation groups (lysine can be one, two, and trimethylation, arginine can be single, symmetric, and asymmetric dimethylation). Histone lysine methylation usually occurs at histone H3 4,9,27,36,79 and histone H4 20th lysine; Arginine methylation usually occurs at histone H3 at positions 2, 8, 17, 26 and histone H4 at position 3. Protein methylase (HMT) and histone demethylase (HDM) are the main regulators of methylation modification. Cellular location / Images Dot Blot Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Immunogen peptide quantity: 4 ng, 20 ng, 100 ngExposure time: 60 sThe list of peptides is included in the table below. WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: HeLa, 2: SHSY-5Y, 3: NIH/3T3, 4: BRLProtein loading quantity: 20 μgExposure time: 60 sPredicted band size: MultipleObserved band size: Multiple