anti-asymmetric dimethyl-histone h3 (arg2) rabbit pab

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Anti-Asymmetric Dimethyl-Histone H3 (Arg2) Rabbit pAb Clone Number: / Host: Rabbit Clonality: Polyclonal Applications： WB ChIP Reactivity: Human, Mouse, Rat, Monkey Synonyms: H3R2me2a Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H3R2me2a UniProt ID P68431 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Mouse, Rat, African green monkey ChIP 6 μg/5×106 cells Human Properties Purity Protein A and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histone post-translational modifications (PTMs) are key mechanisms of epigenetics that modulate chromatin structures, termed as “histone code”. The PTMs on histone including acetylation, methylation, phosphorylation and novel acylations directly affect the accessibility of chromatin to transcription factors and other epigenetic regulators, altering genome stability, gene transcription, etc. Histone methylation occurs primarily at lysine and arginine residues on the amino terminal of core histones. Methylation of histones can either increase or decrease transcription of genes, depending on which amino acids (Lys or Arg) in the histones are methylated and how many methyl groups are attached (mono-, di-, Trimethylation on Lys, mono-di-symmetric/asymmetric methylation on Arg). Mostly, lysine methylation occurs primarily on histone H3 Lys4, 9, 27, 36, 79 and H4 Lys20, while Arginine methylation occurs primarily on histone H3 Arg2, 8, 17, 26 and H4 Arg3. Histone methylases (HMTs) and histone demethylases (HDMs) are major regulating factors. Cellular location Nucleus Images Dot Blot Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgGH&L(HRP Conjugate)Immunogen peptide quantity: 1 ng, 4 ng, 16ngExposure time: 60 secondsThe list of peptides is included in the table below. WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgGH&L(HRP Conjugate) Lysate: HeLa, C2C12, COS-7, rH3 Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 17 kDa Observed band size: 17 kDa ChIP Cell type: HeLa Cross-linking conditions: No cross-linkingAmount of chromatin per IP: 5×106 cells Amount of Ab per IP: 6 μg Beads type and amount per IP: 50 μL of Protein A MagBeads Description: Chromatin immunoprecipitations were performed with 6μgof normal rabbit IgG as a negative control. Real timequantitative PCR was performed on immunoprecipitated DNA using primers specificfor the human RPL30 Exon 3 and TUBBP10. The data are presented as enrichment of eachsample relative to total amount of input chromatinat each amplicon. :
Anti-Asymmetric Dimethyl-Histone H3 (Arg2) Rabbit pAb Clone Number: / Host: Rabbit Clonality: Polyclonal Applications： WB ChIP Reactivity: Human, Mouse, Rat, Monkey Synonyms: H3R2me2a Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H3R2me2a UniProt ID P68431 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Mouse, Rat, African green monkey ChIP 6 μg/5×106 cells Human Properties Purity Protein A and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histone post-translational modifications (PTMs) are key mechanisms of epigenetics that modulate chromatin structures, termed as “histone code”. The PTMs on histone including acetylation, methylation, phosphorylation and novel acylations directly affect the accessibility of chromatin to transcription factors and other epigenetic regulators, altering genome stability, gene transcription, etc. Histone methylation occurs primarily at lysine and arginine residues on the amino terminal of core histones. Methylation of histones can either increase or decrease transcription of genes, depending on which amino acids (Lys or Arg) in the histones are methylated and how many methyl groups are attached (mono-, di-, Trimethylation on Lys, mono-di-symmetric/asymmetric methylation on Arg). Mostly, lysine methylation occurs primarily on histone H3 Lys4, 9, 27, 36, 79 and H4 Lys20, while Arginine methylation occurs primarily on histone H3 Arg2, 8, 17, 26 and H4 Arg3. Histone methylases (HMTs) and histone demethylases (HDMs) are major regulating factors. Cellular location Nucleus Images Dot Blot Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgGH&L(HRP Conjugate)Immunogen peptide quantity: 1 ng, 4 ng, 16ngExposure time: 60 secondsThe list of peptides is included in the table below. WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgGH&L(HRP Conjugate) Lysate: HeLa, C2C12, COS-7, rH3 Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 17 kDa Observed band size: 17 kDa ChIP Cell type: HeLa Cross-linking conditions: No cross-linkingAmount of chromatin per IP: 5×106 cells Amount of Ab per IP: 6 μg Beads type and amount per IP: 50 μL of Protein A MagBeads Description: Chromatin immunoprecipitations were performed with 6μgof normal rabbit IgG as a negative control. Real timequantitative PCR was performed on immunoprecipitated DNA using primers specificfor the human RPL30 Exon 3 and TUBBP10. The data are presented as enrichment of eachsample relative to total amount of input chromatinat each amplicon.