anti-β-hydroxybutyryllysine rabbit pab

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Anti-β-Hydroxybutyryllysine Rabbit pAb Clone Number: / Host: Rabbit Clonality: Polyclonal Applications： WB ICC/IF Reactivity: All Synonyms: Kbhb Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms Kbhb UniProt ID / Immunogen β-Hydroxybutylated lysine peptides MW (kDa) Multiple Specificity Anti-β-Hydroxybutyllysine Rabbit pAb detects proteins post-translationally modified by β-hydroxybutylation on lysine residues. This pan antibody recognizes β-hydroxybutylated lysine independent of its surrounding sequences. Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 All ICC/IF 1:50 Human Properties Purity Protein A and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histones are subject to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitination, etc. β-Hydroxybutyrylation of lysine (Kbhb) is a newly identified histone marker that is enriched in active gene promoters. Histone Kbhb marks are dramatically induced in response to elevated β-hydroxybutyrate levels in cultured cells and in livers from mice subjected to prolonged fasting or streptozotocin-induced diabetic ketoacidosis. Histone β-hydroxybutyrylation represents a new epigenetic regulatory mark that couples metabolism to gene expression, offering a new avenue to study chromatin regulation and diverse functions of β-hydroxybutyrate in the context of important human pathophysiological states, including diabetes, epilepsy, and neoplasia. Cellular location / Images Dot Blot Peptide/protein amount: 10 ngBlocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 60 seconds The list of peptides and proteins used in the experiment is provided in the table below. WB Lysates: HeLa, C2C12, COS, (-): HeLa, (+): HeLa + sodium 3-hydroxybutyrate (100 mM, 72 hours) Protein loading amount: 20 μg Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugated) Exposure time: 60 seconds Predicted band size: Multiple Observed band size: Multiple ICC/IF Samples: (A) HeLa; (B) HeLa + sodium 3-hydroxybutyrate (100 mM, 72 hours) Fixative: 4% Paraformaldehyde Permeabilization: 0.1% Triton X-100 Primary Ab dilution: 1:50 Primary incubation condition: 4°C overnight Secondary Ab: Goat Anti-Rabbit IgG Nuclear counter stain: DAPI (blue) Description: The green color represents the positive signal observed with :
Anti-β-Hydroxybutyryllysine Rabbit pAb Clone Number: / Host: Rabbit Clonality: Polyclonal Applications： WB ICC/IF Reactivity: All Synonyms: Kbhb Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms Kbhb UniProt ID / Immunogen β-Hydroxybutylated lysine peptides MW (kDa) Multiple Specificity Anti-β-Hydroxybutyllysine Rabbit pAb detects proteins post-translationally modified by β-hydroxybutylation on lysine residues. This pan antibody recognizes β-hydroxybutylated lysine independent of its surrounding sequences. Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 All ICC/IF 1:50 Human Properties Purity Protein A and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histones are subject to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitination, etc. β-Hydroxybutyrylation of lysine (Kbhb) is a newly identified histone marker that is enriched in active gene promoters. Histone Kbhb marks are dramatically induced in response to elevated β-hydroxybutyrate levels in cultured cells and in livers from mice subjected to prolonged fasting or streptozotocin-induced diabetic ketoacidosis. Histone β-hydroxybutyrylation represents a new epigenetic regulatory mark that couples metabolism to gene expression, offering a new avenue to study chromatin regulation and diverse functions of β-hydroxybutyrate in the context of important human pathophysiological states, including diabetes, epilepsy, and neoplasia. Cellular location / Images Dot Blot Peptide/protein amount: 10 ngBlocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 60 seconds The list of peptides and proteins used in the experiment is provided in the table below. WB Lysates: HeLa, C2C12, COS, (-): HeLa, (+): HeLa + sodium 3-hydroxybutyrate (100 mM, 72 hours) Protein loading amount: 20 μg Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugated) Exposure time: 60 seconds Predicted band size: Multiple Observed band size: Multiple ICC/IF Samples: (A) HeLa; (B) HeLa + sodium 3-hydroxybutyrate (100 mM, 72 hours) Fixative: 4% Paraformaldehyde Permeabilization: 0.1% Triton X-100 Primary Ab dilution: 1:50 Primary incubation condition: 4°C overnight Secondary Ab: Goat Anti-Rabbit IgG Nuclear counter stain: DAPI (blue) Description: The green color represents the positive signal observed with