anti-β-hydroxybutyryllysine rabbit mab

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Recombinant Rabbit Monoclonal Anti-β-Hydroxybutyryllysine Rabbit mAb Clone Number: 7H1L3 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P ICC/IF Reactivity: All Synonyms: Kbhb Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms Kbhb UniProt ID / Immunogen β-Hydroxybutylated lysine peptides MW (kDa) Multiple Specificity Anti-β-Hydroxybutyllysine Rabbit mAb detects proteins post-translationally modified by β-hydroxybutylation on lysine residues. This pan antibody recognizes β-hydroxybutylated lysine independent of its surrounding sequences. Product Usage Information Applications Dilution Recommended Species WB 1:1000 – 1:2000 All IHC-P 1:100 – 1:200 Human ICC/IF 1:200 – 1:1000 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histones are subject to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitination, etc. β-Hydroxybutyrylation of lysine (Kbhb) is a newly identified histone marker that is enriched in active gene promoters. Histone Kbhb marks are dramatically induced in response to elevated β-hydroxybutyrate levels in cultured cells and in livers from mice subjected to prolonged fasting or streptozotocin-induced diabetic ketoacidosis. Histone β-hydroxybutyrylation represents a new epigenetic regulatory mark that couples metabolism to gene expression, offering a new avenue to study chromatin regulation and diverse functions of β-hydroxybutyrate in the context of important human pathophysiological states, including diabetes, epilepsy, and neoplasia. Cellular location / Images Dot Blot Peptide amount: 1 ng, 4 ng, 16 ngBlocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 15 seconds The list of peptides used in the experiment is provided in the table below. WB Lysates: (-): HeLa; (+): HeLa + sodium 3-hydroxybutyrate (50 mM, 72 hours) Protein loading amount: 20 μg Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 30 seconds Predicted band size: Multiple Observed band size: Multiple IHC-P Tissue: Human kidneySection type: Formalin fixed & paraffin-embedded sectionRetrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0Primary Ab dilution: 1:1000 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use) Counter stain: Hematoxylin (blue) Description: The brown color represents the positive signal observed with :
Recombinant Rabbit Monoclonal Anti-β-Hydroxybutyryllysine Rabbit mAb Clone Number: 7H1L3 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P ICC/IF Reactivity: All Synonyms: Kbhb Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms Kbhb UniProt ID / Immunogen β-Hydroxybutylated lysine peptides MW (kDa) Multiple Specificity Anti-β-Hydroxybutyllysine Rabbit mAb detects proteins post-translationally modified by β-hydroxybutylation on lysine residues. This pan antibody recognizes β-hydroxybutylated lysine independent of its surrounding sequences. Product Usage Information Applications Dilution Recommended Species WB 1:1000 – 1:2000 All IHC-P 1:100 – 1:200 Human ICC/IF 1:200 – 1:1000 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histones are subject to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitination, etc. β-Hydroxybutyrylation of lysine (Kbhb) is a newly identified histone marker that is enriched in active gene promoters. Histone Kbhb marks are dramatically induced in response to elevated β-hydroxybutyrate levels in cultured cells and in livers from mice subjected to prolonged fasting or streptozotocin-induced diabetic ketoacidosis. Histone β-hydroxybutyrylation represents a new epigenetic regulatory mark that couples metabolism to gene expression, offering a new avenue to study chromatin regulation and diverse functions of β-hydroxybutyrate in the context of important human pathophysiological states, including diabetes, epilepsy, and neoplasia. Cellular location / Images Dot Blot Peptide amount: 1 ng, 4 ng, 16 ngBlocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 15 seconds The list of peptides used in the experiment is provided in the table below. WB Lysates: (-): HeLa; (+): HeLa + sodium 3-hydroxybutyrate (50 mM, 72 hours) Protein loading amount: 20 μg Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 30 seconds Predicted band size: Multiple Observed band size: Multiple IHC-P Tissue: Human kidneySection type: Formalin fixed & paraffin-embedded sectionRetrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0Primary Ab dilution: 1:1000 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use) Counter stain: Hematoxylin (blue) Description: The brown color represents the positive signal observed with