anti-β-hydroxybutyryl-histone h4 (lys12) rabbit mab

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Recombinant Rabbit Monoclonal Anti-β-Hydroxybutyryl-Histone H4 (Lys12) Rabbit mAb Clone Number: 9H1L2 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB ICC/IF ChIP Reactivity: Human Synonyms: H4K12bhb Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H4K12bhb UniProt ID P62805 Immunogen β-Hydroxybutylated human histone H4 (Lys12) peptide MW (kDa) 11 Specificity Anti-β-Hydroxybutyryl-Histone H4 (Lys12) Rabbit mAb detects histone H4 only when it is β-hydroxybutyrylated at Lys12. Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human ICC/IF 1:50 Human ChIP 6 μg per 5×106 cells Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histones are subject to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitination, etc. β-Hydroxybutyrylation of lysine (Kbhb) is a newly identified histone marker that is enriched in active gene promoters. Histone Kbhb marks are dramatically induced in response to elevated β-hydroxybutyrate levels in cultured cells and in livers from mice subjected to prolonged fasting or streptozotocin-induced diabetic ketoacidosis. Histone β-hydroxybutyrylation represents a new epigenetic regulatory mark that couples metabolism to gene expression, offering a new avenue to study chromatin regulation and diverse functions of β-hydroxybutyrate in the context of important human pathophysiological states, including diabetes, epilepsy, and neoplasia. Cellular location Nucleus Images Dot Blot Peptide amount: 1 ng, 4 ng, 16 ng, 64 ng Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:10000 Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 30 seconds The list of peptides used in the experiment is provided in the table below. WB Lysate: (-): HeLa; (+): HeLa + sodium 3-hydroxybutyrate (50 mM, 72 hours) Protein loading amount: 20 μg Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 30 seconds Predicted band size: 11 kDa Observed band size: 11 kDa ICC/IF Cell line: (A) HeLa; (B) HeLa + sodium 3-hydroxybutyrate (50 mM, 72 hours) Fixative: 4% Paraformaldehyde Permeabilization: 0.1% Triton X-100 Primary Ab dilution: 1:50 Primary incubation condition: 4°C overnight Secondary Ab: Goat Anti-Rabbit IgG Nuclear counter stain: DAPI (blue) Counter stain: Tubulin (red)Description: The green color represents the positive signal observed with :
Recombinant Rabbit Monoclonal Anti-β-Hydroxybutyryl-Histone H4 (Lys12) Rabbit mAb Clone Number: 9H1L2 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB ICC/IF ChIP Reactivity: Human Synonyms: H4K12bhb Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H4K12bhb UniProt ID P62805 Immunogen β-Hydroxybutylated human histone H4 (Lys12) peptide MW (kDa) 11 Specificity Anti-β-Hydroxybutyryl-Histone H4 (Lys12) Rabbit mAb detects histone H4 only when it is β-hydroxybutyrylated at Lys12. Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human ICC/IF 1:50 Human ChIP 6 μg per 5×106 cells Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histones are subject to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitination, etc. β-Hydroxybutyrylation of lysine (Kbhb) is a newly identified histone marker that is enriched in active gene promoters. Histone Kbhb marks are dramatically induced in response to elevated β-hydroxybutyrate levels in cultured cells and in livers from mice subjected to prolonged fasting or streptozotocin-induced diabetic ketoacidosis. Histone β-hydroxybutyrylation represents a new epigenetic regulatory mark that couples metabolism to gene expression, offering a new avenue to study chromatin regulation and diverse functions of β-hydroxybutyrate in the context of important human pathophysiological states, including diabetes, epilepsy, and neoplasia. Cellular location Nucleus Images Dot Blot Peptide amount: 1 ng, 4 ng, 16 ng, 64 ng Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:10000 Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 30 seconds The list of peptides used in the experiment is provided in the table below. WB Lysate: (-): HeLa; (+): HeLa + sodium 3-hydroxybutyrate (50 mM, 72 hours) Protein loading amount: 20 μg Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 30 seconds Predicted band size: 11 kDa Observed band size: 11 kDa ICC/IF Cell line: (A) HeLa; (B) HeLa + sodium 3-hydroxybutyrate (50 mM, 72 hours) Fixative: 4% Paraformaldehyde Permeabilization: 0.1% Triton X-100 Primary Ab dilution: 1:50 Primary incubation condition: 4°C overnight Secondary Ab: Goat Anti-Rabbit IgG Nuclear counter stain: DAPI (blue) Counter stain: Tubulin (red)Description: The green color represents the positive signal observed with