anti-von willebrand factor rabbit mab

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Recombinant Rabbit Monoclonal Anti-Von Willebrand Factor Rabbit mAb Clone Number: JRMR-10117(2)-19 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P Reactivity: Human, Mouse Synonyms: vWF, F8VWF, VWD, Von Willebrand antigen II Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms vWF, F8VWF, VWD, Von Willebrand antigen II UniProt ID Q8CIZ8 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:5000-1:10000 Mouse IHC-P 1:100 – 1:1000 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background VWF (Von Willebrand factor) is a multimeric plasma glycoprotein that promotes adhesion of platelets to sites of vascular injury. Mature circulating VWF is made up of disulfide-bonded multimers that are in a complex with factor VIII. VWF is stored in secretory Weibel-Palade bodies in endothelial cells. It is synthesized as a large precursor protein and undergoes extensive posttranslational modifications including dimerization in the endoplasmic reticulum followed by cleavage of the pro-peptide and multimerization in the Golgi apparatus. VWF is important in hemostasis, and genetic defects in the structure and modification of VWF can cause von Willebrand disease (VWD), the most common congenital bleeding disorder in humans. Alternatively, increased levels of VWF have been shown to be involved in acute coronary thrombosis and are a clinical risk marker for atherosclerosis. Cellular location Secreted Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:10000Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: Mouse plateletProtein loading amount: 20 μgExposure time: 60 secondsPredicted band size: 309 kDa Observed band size: 309 kDa IHC-P Tissue: Human tonsilSection type: Formalin-fixed & paraffin-embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0Primary Ab dilution: 1:100Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)Counter stain: Hematoxylin (blue)Description: The brown color represents the positive signal observed with :
Recombinant Rabbit Monoclonal Anti-Von Willebrand Factor Rabbit mAb Clone Number: JRMR-10117(2)-19 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P Reactivity: Human, Mouse Synonyms: vWF, F8VWF, VWD, Von Willebrand antigen II Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms vWF, F8VWF, VWD, Von Willebrand antigen II UniProt ID Q8CIZ8 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:5000-1:10000 Mouse IHC-P 1:100 – 1:1000 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background VWF (Von Willebrand factor) is a multimeric plasma glycoprotein that promotes adhesion of platelets to sites of vascular injury. Mature circulating VWF is made up of disulfide-bonded multimers that are in a complex with factor VIII. VWF is stored in secretory Weibel-Palade bodies in endothelial cells. It is synthesized as a large precursor protein and undergoes extensive posttranslational modifications including dimerization in the endoplasmic reticulum followed by cleavage of the pro-peptide and multimerization in the Golgi apparatus. VWF is important in hemostasis, and genetic defects in the structure and modification of VWF can cause von Willebrand disease (VWD), the most common congenital bleeding disorder in humans. Alternatively, increased levels of VWF have been shown to be involved in acute coronary thrombosis and are a clinical risk marker for atherosclerosis. Cellular location Secreted Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:10000Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: Mouse plateletProtein loading amount: 20 μgExposure time: 60 secondsPredicted band size: 309 kDa Observed band size: 309 kDa IHC-P Tissue: Human tonsilSection type: Formalin-fixed & paraffin-embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0Primary Ab dilution: 1:100Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)Counter stain: Hematoxylin (blue)Description: The brown color represents the positive signal observed with