anti-vista rabbit mab

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Recombinant Rabbit Monoclonal Anti-VISTA Rabbit mAb Clone Number: / Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB Reactivity: Human, Mouse Synonyms: Platelet receptor Gi24, Sisp-1, VSIR Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms Platelet receptor Gi24, Sisp-1, VSIR UniProt ID Q9H7M9 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Mouse Properties Purity Protein A and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background VISTA is a negative checkpoint control protein that regulates T cell activation and immune responses. VISTA, which contains a single Ig-like V-type domain, a transmembrane domain, and an intracellular domain, has sequence similarity to both the B7 and CD28 family members. Although primarily expressed by myeloid cells, VISTA is also expressed by CD4+, CD8+, and FoxP3+ T-cells. Thus, VISTA is described as both a ligand and a receptor. Blocking VISTA induces T-cell activation and proliferation, and potentiates disease severity in the EAE model. Furthermore, genetic deletion of VISTA in mice leads to spontaneous T-cell activation and chronic inflammation. In mouse models of cancer, neutralization of VISTA enhances T-cell proliferation and effector function and increases tumor infiltration, suggesting VISTA blockade could be an effective strategy for tumor immunotherapy. Cellular location Membrane Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb(HRP Conjugate)Lysate: 1: MCF7, 2: HeLa, 3: Jurkat, 4: Human PBMC, 5: NIH/3T3, 6: RAW264.7Protein loading quantity: 20 μgExposure time: 30 sPredicted band size: 34 kDaObserved band size: 45-70 kDa :
Recombinant Rabbit Monoclonal Anti-VISTA Rabbit mAb Clone Number: / Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB Reactivity: Human, Mouse Synonyms: Platelet receptor Gi24, Sisp-1, VSIR Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms Platelet receptor Gi24, Sisp-1, VSIR UniProt ID Q9H7M9 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Mouse Properties Purity Protein A and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background VISTA is a negative checkpoint control protein that regulates T cell activation and immune responses. VISTA, which contains a single Ig-like V-type domain, a transmembrane domain, and an intracellular domain, has sequence similarity to both the B7 and CD28 family members. Although primarily expressed by myeloid cells, VISTA is also expressed by CD4+, CD8+, and FoxP3+ T-cells. Thus, VISTA is described as both a ligand and a receptor. Blocking VISTA induces T-cell activation and proliferation, and potentiates disease severity in the EAE model. Furthermore, genetic deletion of VISTA in mice leads to spontaneous T-cell activation and chronic inflammation. In mouse models of cancer, neutralization of VISTA enhances T-cell proliferation and effector function and increases tumor infiltration, suggesting VISTA blockade could be an effective strategy for tumor immunotherapy. Cellular location Membrane Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb(HRP Conjugate)Lysate: 1: MCF7, 2: HeLa, 3: Jurkat, 4: Human PBMC, 5: NIH/3T3, 6: RAW264.7Protein loading quantity: 20 μgExposure time: 30 sPredicted band size: 34 kDaObserved band size: 45-70 kDa