anti-vimentin rabbit mab

Vapor Pressure :
Recombinant Rabbit Monoclonal Anti-Vimentin Rabbit mAb Clone Number: JRMR-10214-73 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P ICC/IF FC IP mIHC-P Reactivity: Human, Mouse, Rat Synonyms: VIM Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms VIM UniProt ID P08670 Immunogen Recombinant human Vimentin fragment MW (kDa) 54 Specificity Anti-Vimentin Rabbit mAb recognizes endogeneous level of Vimentin protein. Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Mouse, Rat IHC-P 1:100 – 1:500 Human, Mouse, Rat ICC/IF 1:50 Human, Mouse FC 1:50 – 1:100 Human IP 1:25 – 1:100 Human mIHC-P 1:500 – 1:1000 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2. Cellular location Cytoskeleton, Cytoplasm Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: HeLa, HEK-293, Jurkat, Rat brain, Mouse brainProtein loading quantity: 20 μgExposure time: 3 sPredicted band size: 54 kDaObserved band size: 54 kDa IHC-P Tissue: Human cervical carcinomaSection type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:500Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)Counter stain: Hematoxylin (Blue)Description: The brown color represents the positive signal observed with :
Recombinant Rabbit Monoclonal Anti-Vimentin Rabbit mAb Clone Number: JRMR-10214-73 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P ICC/IF FC IP mIHC-P Reactivity: Human, Mouse, Rat Synonyms: VIM Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms VIM UniProt ID P08670 Immunogen Recombinant human Vimentin fragment MW (kDa) 54 Specificity Anti-Vimentin Rabbit mAb recognizes endogeneous level of Vimentin protein. Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Mouse, Rat IHC-P 1:100 – 1:500 Human, Mouse, Rat ICC/IF 1:50 Human, Mouse FC 1:50 – 1:100 Human IP 1:25 – 1:100 Human mIHC-P 1:500 – 1:1000 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2. Cellular location Cytoskeleton, Cytoplasm Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: HeLa, HEK-293, Jurkat, Rat brain, Mouse brainProtein loading quantity: 20 μgExposure time: 3 sPredicted band size: 54 kDaObserved band size: 54 kDa IHC-P Tissue: Human cervical carcinomaSection type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:500Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)Counter stain: Hematoxylin (Blue)Description: The brown color represents the positive signal observed with