anti-vasp rabbit mab

Vapor Pressure :
Recombinant Rabbit Monoclonal Anti-VASP Rabbit mAb Clone Number: JMMR-3193 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IP Reactivity: Human Synonyms: Vasodilator-stimulated phosphoprotein Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG/kappa Conjugate Unconjugated Synonyms Vasodilator-stimulated phosphoprotein UniProt ID P50552 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human IP 1:50 – 1:100 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Vasodilator-stimulated phosphoprotein (VASP) was originally characterized as a substrate of both cGMP- and cAMP-dependent kinases (PKG and PKA, or cGPK and cAPK, respectively). It is now believed that VASP belongs to the Ena/VASP family of adaptor proteins linking the cytoskeletal system to the signal transduction pathways and that it functions in cytoskeletal organization, fibroblast migration, platelet activation and axon guidance. Three phosphorylation sites, Ser157, Ser239, and Thr278, have been identified. Ser239 is the major PKG phosphorylation site while Ser157 is the major PKA phosphorylation site. Evidence suggests that VASP phosphorylation reduces its association with actin and has a negative effect on actin polymerization. Phosphorylation at Ser239 of VASP is a useful marker for monitoring PKG activation and signaling. Cellular location Cytoplasm, Cytoskeleton, Cell junction Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate) Lysate: 1: HeLa, 2: THP-1 Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 40 kDa Observed band size: 46 kDa IP IP of HeLa cells extractsIP Ab incubation condition: :
Recombinant Rabbit Monoclonal Anti-VASP Rabbit mAb Clone Number: JMMR-3193 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IP Reactivity: Human Synonyms: Vasodilator-stimulated phosphoprotein Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG/kappa Conjugate Unconjugated Synonyms Vasodilator-stimulated phosphoprotein UniProt ID P50552 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human IP 1:50 – 1:100 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Vasodilator-stimulated phosphoprotein (VASP) was originally characterized as a substrate of both cGMP- and cAMP-dependent kinases (PKG and PKA, or cGPK and cAPK, respectively). It is now believed that VASP belongs to the Ena/VASP family of adaptor proteins linking the cytoskeletal system to the signal transduction pathways and that it functions in cytoskeletal organization, fibroblast migration, platelet activation and axon guidance. Three phosphorylation sites, Ser157, Ser239, and Thr278, have been identified. Ser239 is the major PKG phosphorylation site while Ser157 is the major PKA phosphorylation site. Evidence suggests that VASP phosphorylation reduces its association with actin and has a negative effect on actin polymerization. Phosphorylation at Ser239 of VASP is a useful marker for monitoring PKG activation and signaling. Cellular location Cytoplasm, Cytoskeleton, Cell junction Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate) Lysate: 1: HeLa, 2: THP-1 Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 40 kDa Observed band size: 46 kDa IP IP of HeLa cells extractsIP Ab incubation condition: