anti-vamp3 mouse mab

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Recombinant Anti-VAMP3 Mouse mAb Clone Number: JMMR-2753 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB IHC-P Reactivity: Human, Mouse, Rat Synonyms: Cellubrevin, Synaptobrevin-3 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG2b/kappa Conjugate Unconjugated Synonyms Cellubrevin, Synaptobrevin-3 UniProt ID Q15836 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:2000-1:10000 Human, Mouse, Rat IHC-P 1:100 – 1:500 Mouse Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background VAMP3, also named as cellubrevin or synaptobrevin 3, is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. VAMP3 is a v-SNARE (soluble NSF-attachment protein receptor). Characterized by a common sequence called the SNARE motif, SNARE proteins are involved in membrane fusion and vesicular transport. VAMP3 resides in recycling endosomes and endosome-derived transport vesicles, involved in regulating membrane traffic. It is implicated in recycling of transferrin receptors, secretion of alpha-granules in platelets, and membrane trafficking during cell migration. Cellular location Membrane Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:10000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgGH&L(HRP Conjugate) Lysate: 1: SK-MBL-28, 2: Mouse brain, 3: Rat liver(-), 4: Rat brain Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 11 kDa Observed band size: 13, 17 kDa IHC-P Tissue: Mouse testis Section type: Formalin-fixed & Paraffin-embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary Ab dilution: 1:500 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use) Counter stain: Hematoxylin (Blue) Description: The brown color represents the positive signal observed with :
Recombinant Anti-VAMP3 Mouse mAb Clone Number: JMMR-2753 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB IHC-P Reactivity: Human, Mouse, Rat Synonyms: Cellubrevin, Synaptobrevin-3 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG2b/kappa Conjugate Unconjugated Synonyms Cellubrevin, Synaptobrevin-3 UniProt ID Q15836 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:2000-1:10000 Human, Mouse, Rat IHC-P 1:100 – 1:500 Mouse Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background VAMP3, also named as cellubrevin or synaptobrevin 3, is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. VAMP3 is a v-SNARE (soluble NSF-attachment protein receptor). Characterized by a common sequence called the SNARE motif, SNARE proteins are involved in membrane fusion and vesicular transport. VAMP3 resides in recycling endosomes and endosome-derived transport vesicles, involved in regulating membrane traffic. It is implicated in recycling of transferrin receptors, secretion of alpha-granules in platelets, and membrane trafficking during cell migration. Cellular location Membrane Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:10000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgGH&L(HRP Conjugate) Lysate: 1: SK-MBL-28, 2: Mouse brain, 3: Rat liver(-), 4: Rat brain Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 11 kDa Observed band size: 13, 17 kDa IHC-P Tissue: Mouse testis Section type: Formalin-fixed & Paraffin-embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary Ab dilution: 1:500 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use) Counter stain: Hematoxylin (Blue) Description: The brown color represents the positive signal observed with