anti-ubiquityl-histone h2b (lys120) rabbit mab

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Recombinant Rabbit Monoclonal Anti-Ubiquityl-Histone H2B (Lys120) Rabbit mAb Clone Number: 15H3L2 Host: Rabbit Clonality: Monoclonal Applications： WB IHC-P Reactivity: Human, Mouse, Rat Synonyms: H2BK120ub Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H2BK120ub UniProt ID P62807 Immunogen Ubiquitinated human histone H2B (Lys120) peptide MW (kDa) 23 Specificity Anti-Ubiquityl-Histone H2B (Lys120) Rabbit mAb detects histone H2B only when it is ubiquitinated at Lys120. This antibody has been shown to selectively recognize ubiquitinated H2B peptide at Lys120, but not the unmodified peptide. Product Usage Information Applications Dilution Recommended Species WB 1:500-1:2000 Human, Mouse, Rat IHC-P 1: 200-1:1000 Human, Mouse, Rat Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby is critical for transcription regulation, DNA repair, DNA replication, and chromosomal stability. Mono-ubiquitination of Histone H2B at Lys120 (H2BK120ub) is a key histone modification that plays critical roles in gene transcriptional regulation and higher order chromatin organization in many species. Cellular location Nucleus Images Dot Blot Blocking buffer: 5% NFDM/TBSTPrimary ab dilution: 1:2000Primary ab incubation condition: 2 hours at room temperatureSecondary ab: Goat Anti-Rabbit IgG H&L (HRP)Peptide amount: 1 ng, 4 ng, 16 ngExposure time: 60 sThe list of peptides is included in the table below. WB Blocking buffer: 5% NFDM/TBSTPrimary ab dilution: 1:2000Primary ab incubation condition: 2 hours at room temperatureSecondary ab: Goat Anti-Rabbit IgG H&L (HRP)Lysate: HeLa, N2a, BRL, Mouse liver, Mouse kidneyProtein loading amount: 20 μgExposure time: 30 sPredicted MW: 23 kDaObserved MW: 23 kDa IHC-P Tissue: Human lung squamous carcinomaSection type: Formalin fixed & paraffin-embedded sectionRetrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0Primary ab dilution: 1:1000Primary ab incubation condition: 1 hour at room temperatureSecondary ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use)Counter stain: Hematoxylin (blue)Comment: Color brown is the positive signal for :
Recombinant Rabbit Monoclonal Anti-Ubiquityl-Histone H2B (Lys120) Rabbit mAb Clone Number: 15H3L2 Host: Rabbit Clonality: Monoclonal Applications： WB IHC-P Reactivity: Human, Mouse, Rat Synonyms: H2BK120ub Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H2BK120ub UniProt ID P62807 Immunogen Ubiquitinated human histone H2B (Lys120) peptide MW (kDa) 23 Specificity Anti-Ubiquityl-Histone H2B (Lys120) Rabbit mAb detects histone H2B only when it is ubiquitinated at Lys120. This antibody has been shown to selectively recognize ubiquitinated H2B peptide at Lys120, but not the unmodified peptide. Product Usage Information Applications Dilution Recommended Species WB 1:500-1:2000 Human, Mouse, Rat IHC-P 1: 200-1:1000 Human, Mouse, Rat Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby is critical for transcription regulation, DNA repair, DNA replication, and chromosomal stability. Mono-ubiquitination of Histone H2B at Lys120 (H2BK120ub) is a key histone modification that plays critical roles in gene transcriptional regulation and higher order chromatin organization in many species. Cellular location Nucleus Images Dot Blot Blocking buffer: 5% NFDM/TBSTPrimary ab dilution: 1:2000Primary ab incubation condition: 2 hours at room temperatureSecondary ab: Goat Anti-Rabbit IgG H&L (HRP)Peptide amount: 1 ng, 4 ng, 16 ngExposure time: 60 sThe list of peptides is included in the table below. WB Blocking buffer: 5% NFDM/TBSTPrimary ab dilution: 1:2000Primary ab incubation condition: 2 hours at room temperatureSecondary ab: Goat Anti-Rabbit IgG H&L (HRP)Lysate: HeLa, N2a, BRL, Mouse liver, Mouse kidneyProtein loading amount: 20 μgExposure time: 30 sPredicted MW: 23 kDaObserved MW: 23 kDa IHC-P Tissue: Human lung squamous carcinomaSection type: Formalin fixed & paraffin-embedded sectionRetrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0Primary ab dilution: 1:1000Primary ab incubation condition: 1 hour at room temperatureSecondary ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use)Counter stain: Hematoxylin (blue)Comment: Color brown is the positive signal for