anti-trimethyllysine rabbit pab

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Anti-Trimethyllysine Rabbit pAb Clone Number: / Host: Rabbit Clonality: Polyclonal Applications： WB ChIP Reactivity: All Synonyms: Kme3 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms Kme3 UniProt ID / Immunogen Trimethylated lysine peptides MW (kDa) Multiple Specificity Anti-Trimethyl-Lysine Rabbit pAb detects proteins with Trimethylated lysine residues. This pan antibody recognizes Trimethylated lysine independent of its surrounding sequences. Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 All ChIP 6 μg per 5×106 cells Human Properties Purity Protein A and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histone post-translational modifications (PTMs), known as the “histone code”, are key mechanisms of epigenetics that modulate chromatin structures. The PTMs on histone including acetylation, methylation, phosphorylation and novel acylations directly affect the accessibility of chromatin to transcription factors and other epigenetic regulators, altering genome stability and gene transcription. Histone methylation primarily occurs at lysine and arginine residues on the amino terminus of core histones. The methylation of histones can either enhance or repress gene transcription, depending on the specific amino acids (Lys or Arg) being methylated and the number of attached methyl groups (mono-, di-, or Trimethylation for Lys; mono-di-symmetric/asymmetric methylation for Arg). Lysine methylation is commonly observed at histone H3 Lys residues 4, 9, 27, 36, 79, and histone H4 Lys20, while Arginine methylation predominantly occurs at histone H3 Arg residues 2, 8, 17, 26, and histone H4 Arg3. The regulation of histone methylation is primarily governed by histone methylases (HMTs) and histone demethylases (HDMs). Cellular location / Images Dot Blot Protein amount: 4 ng, 20 ng, 100 ng, 500 ngBlocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 60 seconds The list of proteins used in the experiment is provided in the table below. WB Lysates: HeLaProtein loading amount: 20 μg Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugated) Exposure time: 60 seconds Predicted band size: Multiple Observed band size: Multiple ChIP Sample: HeLa cells + sodium butyrate (5 mM, 24 hours)Cross-linking conditions: No cross-linking Amount of chromatin per IP: 5×106 cells Amount of Ab per IP: 6 μg Beads type and amount per IP: 50 μL of Protein A MagBeads Description: Chromatin immunoprecipitations were performed with 6 μg of normal rabbit IgG as a negative control. The immunoprecipitated DNA was quantified by real-time PCR using primers specific for the human GAPDH CDS, RPL30, LDHA promoter, LDHA CDS, and TUBBP10 regions. The data are presented as enrichment of each sample relative to total amount of input at each amplicon. :
Anti-Trimethyllysine Rabbit pAb Clone Number: / Host: Rabbit Clonality: Polyclonal Applications： WB ChIP Reactivity: All Synonyms: Kme3 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms Kme3 UniProt ID / Immunogen Trimethylated lysine peptides MW (kDa) Multiple Specificity Anti-Trimethyl-Lysine Rabbit pAb detects proteins with Trimethylated lysine residues. This pan antibody recognizes Trimethylated lysine independent of its surrounding sequences. Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 All ChIP 6 μg per 5×106 cells Human Properties Purity Protein A and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histone post-translational modifications (PTMs), known as the “histone code”, are key mechanisms of epigenetics that modulate chromatin structures. The PTMs on histone including acetylation, methylation, phosphorylation and novel acylations directly affect the accessibility of chromatin to transcription factors and other epigenetic regulators, altering genome stability and gene transcription. Histone methylation primarily occurs at lysine and arginine residues on the amino terminus of core histones. The methylation of histones can either enhance or repress gene transcription, depending on the specific amino acids (Lys or Arg) being methylated and the number of attached methyl groups (mono-, di-, or Trimethylation for Lys; mono-di-symmetric/asymmetric methylation for Arg). Lysine methylation is commonly observed at histone H3 Lys residues 4, 9, 27, 36, 79, and histone H4 Lys20, while Arginine methylation predominantly occurs at histone H3 Arg residues 2, 8, 17, 26, and histone H4 Arg3. The regulation of histone methylation is primarily governed by histone methylases (HMTs) and histone demethylases (HDMs). Cellular location / Images Dot Blot Protein amount: 4 ng, 20 ng, 100 ng, 500 ngBlocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 60 seconds The list of proteins used in the experiment is provided in the table below. WB Lysates: HeLaProtein loading amount: 20 μg Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugated) Exposure time: 60 seconds Predicted band size: Multiple Observed band size: Multiple ChIP Sample: HeLa cells + sodium butyrate (5 mM, 24 hours)Cross-linking conditions: No cross-linking Amount of chromatin per IP: 5×106 cells Amount of Ab per IP: 6 μg Beads type and amount per IP: 50 μL of Protein A MagBeads Description: Chromatin immunoprecipitations were performed with 6 μg of normal rabbit IgG as a negative control. The immunoprecipitated DNA was quantified by real-time PCR using primers specific for the human GAPDH CDS, RPL30, LDHA promoter, LDHA CDS, and TUBBP10 regions. The data are presented as enrichment of each sample relative to total amount of input at each amplicon.