anti-apoe mouse mab

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Recombinant Anti-ApoE Mouse mAb Clone Number: 11747 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB IHC-P ICC/IF Reactivity: Human, Mouse Synonyms: / Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG1/kappa Conjugate Unconjugated Synonyms / UniProt ID P02649 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:1000 – 1:5000 Human, Mouse IHC-P 1:50 – 1:100 Human, Mouse ICC/IF 1:100 – 1:200 Human Properties Purity Protein G purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background APOE is an apolipoprotein, a protein associating with lipid particles, that mainly functions in lipoprotein-mediated lipid transport between organs via the plasma and interstitial fluids. APOE is a core component of plasma lipoproteins and is involved in their production, conversion and clearance. Apoliproteins are amphipathic molecules that interact both with lipids of the lipoprotein particle core and the aqueous environment of the plasma. As such, APOE associates with chylomicrons, chylomicron remnants, very low density lipoproteins (VLDL) and intermediate density lipoproteins (IDL) but shows a preferential binding to high-density lipoproteins (HDL). It also binds a wide range of cellular receptors including the LDL receptor/LDLR, the LDL receptor-related proteins LRP1, LRP2 and LRP8 and the very low-density lipoprotein receptor/VLDLR that mediate the cellular uptake of the APOE-containing lipoprotein particles. Cellular location Chylomicron, Extracellular matrix, HDL, Secreted, VLDL Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:5000Primary Ab incubation condition: room temperature 2hSecondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)Lysate: Human serumProtein loading quantity: 20 μgExposure time: 10 sPredicted band size: 34-36 kDaObserved band size: 34-36 kDa Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: room temperature 2hSecondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)Lysate: Mouse serumProtein loading quantity: 20 μgExposure time: 10 sPredicted band size: 34-36 kDaObserved band size: 34-36 kDa IHC-P Tissue: Human brainSection type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)Counter stain: Hematoxylin (Blue)Description: The brown color represents the positive signal observed with :
Recombinant Anti-ApoE Mouse mAb Clone Number: 11747 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB IHC-P ICC/IF Reactivity: Human, Mouse Synonyms: / Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG1/kappa Conjugate Unconjugated Synonyms / UniProt ID P02649 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:1000 – 1:5000 Human, Mouse IHC-P 1:50 – 1:100 Human, Mouse ICC/IF 1:100 – 1:200 Human Properties Purity Protein G purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background APOE is an apolipoprotein, a protein associating with lipid particles, that mainly functions in lipoprotein-mediated lipid transport between organs via the plasma and interstitial fluids. APOE is a core component of plasma lipoproteins and is involved in their production, conversion and clearance. Apoliproteins are amphipathic molecules that interact both with lipids of the lipoprotein particle core and the aqueous environment of the plasma. As such, APOE associates with chylomicrons, chylomicron remnants, very low density lipoproteins (VLDL) and intermediate density lipoproteins (IDL) but shows a preferential binding to high-density lipoproteins (HDL). It also binds a wide range of cellular receptors including the LDL receptor/LDLR, the LDL receptor-related proteins LRP1, LRP2 and LRP8 and the very low-density lipoprotein receptor/VLDLR that mediate the cellular uptake of the APOE-containing lipoprotein particles. Cellular location Chylomicron, Extracellular matrix, HDL, Secreted, VLDL Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:5000Primary Ab incubation condition: room temperature 2hSecondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)Lysate: Human serumProtein loading quantity: 20 μgExposure time: 10 sPredicted band size: 34-36 kDaObserved band size: 34-36 kDa Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: room temperature 2hSecondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)Lysate: Mouse serumProtein loading quantity: 20 μgExposure time: 10 sPredicted band size: 34-36 kDaObserved band size: 34-36 kDa IHC-P Tissue: Human brainSection type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)Counter stain: Hematoxylin (Blue)Description: The brown color represents the positive signal observed with