anti-tpx2 rabbit mab

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Recombinant Rabbit Monoclonal Anti-TPX2 Rabbit mAb Clone Number: JRMR-10242-28 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P ICC/IF FC Reactivity: Human, Mouse, Rat Synonyms: C20orf1, C20orf2, DIL2, HCA519 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms C20orf1, C20orf2, DIL2, HCA519 UniProt ID Q9ULW0 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human, Rat IHC-P 1:50 – 1:200 Human, Mouse, Rat ICC/IF 1:50 Human, Mouse FC 1:50 – 1:100 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Spindle assembly factor required for normal assembly of mitotic spindles. Required for normal assembly of microtubules during apoptosis. Required for chromatin and/or kinetochore dependent microtubule nucleation. Mediates AURKA localization to spindle microtubules.Activates AURKA by promoting its autophosphorylation at ‘Thr-288’ and protects this residue against dephosphorylation. TPX2 is inactivated upon binding to importin-alpha .At the onset of mitosis, GOLGA2 interacts with importin-alpha, liberating TPX2 from importin-alpha, allowing TPX2 to activates AURKA kinase and stimulates local microtubule nucleation. Cellular location Nucleus Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: HeLa, 2: Raji, 3: PC-12Protein loading quantity: 20 μgExposure time: 60 sPredicted band size: 86 kDaObserved band size: 100 kDa Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: Raji cell lysate, the TPX2 Rabbit mAb ( :
Recombinant Rabbit Monoclonal Anti-TPX2 Rabbit mAb Clone Number: JRMR-10242-28 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P ICC/IF FC Reactivity: Human, Mouse, Rat Synonyms: C20orf1, C20orf2, DIL2, HCA519 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms C20orf1, C20orf2, DIL2, HCA519 UniProt ID Q9ULW0 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human, Rat IHC-P 1:50 – 1:200 Human, Mouse, Rat ICC/IF 1:50 Human, Mouse FC 1:50 – 1:100 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Spindle assembly factor required for normal assembly of mitotic spindles. Required for normal assembly of microtubules during apoptosis. Required for chromatin and/or kinetochore dependent microtubule nucleation. Mediates AURKA localization to spindle microtubules.Activates AURKA by promoting its autophosphorylation at ‘Thr-288’ and protects this residue against dephosphorylation. TPX2 is inactivated upon binding to importin-alpha .At the onset of mitosis, GOLGA2 interacts with importin-alpha, liberating TPX2 from importin-alpha, allowing TPX2 to activates AURKA kinase and stimulates local microtubule nucleation. Cellular location Nucleus Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: HeLa, 2: Raji, 3: PC-12Protein loading quantity: 20 μgExposure time: 60 sPredicted band size: 86 kDaObserved band size: 100 kDa Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: Raji cell lysate, the TPX2 Rabbit mAb (