anti-tpm4 mouse mab

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Recombinant Anti-TPM4 Mouse mAb Clone Number: JMMR-2747 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB IHC-P ICC/IF Reactivity: Human, Mouse, Rat Synonyms: TM30p1, Tropomyosin-4 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG1/kappa Conjugate Unconjugated Synonyms TM30p1, Tropomyosin-4 UniProt ID P67936 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human, Mouse, Rat IHC-P 1:50 – 1:100 Human, Mouse, Rat ICC/IF 1:50 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background TPM4 (Tropomyosin alpha-4) is a protein that is encoded by the TPM4 gene in human. TPM4 binds to actin filaments in muscle and non-muscle cells, it plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Cellular location Cytoplasm, Cytoskeleton Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate) Lysate: 1: MDA-MB-231, 2: Jurkat Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 29 kDa Observed band size: 30 kDa Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate) Lysate: 1: Rat skeletal muscle, 2: Mouse skeletal muscle Protein loading quantity: 20 μg Exposure time: 10 secondsPredicted band size: 29 kDa Observed band size: 35 kDa IHC-P Tissue: Human cardiac muscle Section type: Formalin-fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use) Counter stain: Hematoxylin (Blue) Description: The brown color represents the positive signal observed with :
Recombinant Anti-TPM4 Mouse mAb Clone Number: JMMR-2747 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB IHC-P ICC/IF Reactivity: Human, Mouse, Rat Synonyms: TM30p1, Tropomyosin-4 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG1/kappa Conjugate Unconjugated Synonyms TM30p1, Tropomyosin-4 UniProt ID P67936 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human, Mouse, Rat IHC-P 1:50 – 1:100 Human, Mouse, Rat ICC/IF 1:50 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background TPM4 (Tropomyosin alpha-4) is a protein that is encoded by the TPM4 gene in human. TPM4 binds to actin filaments in muscle and non-muscle cells, it plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Cellular location Cytoplasm, Cytoskeleton Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate) Lysate: 1: MDA-MB-231, 2: Jurkat Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 29 kDa Observed band size: 30 kDa Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate) Lysate: 1: Rat skeletal muscle, 2: Mouse skeletal muscle Protein loading quantity: 20 μg Exposure time: 10 secondsPredicted band size: 29 kDa Observed band size: 35 kDa IHC-P Tissue: Human cardiac muscle Section type: Formalin-fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use) Counter stain: Hematoxylin (Blue) Description: The brown color represents the positive signal observed with