anti-top3b rabbit mab

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Recombinant Rabbit Monoclonal Anti-TOP3B Rabbit mAb Clone Number: JRMR-10725-50 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB ICC/IF Reactivity: Human Synonyms: DNA topoisomerase 3 beta 1, DNA topoisomerase III beta 1, TOP3B1 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms DNA topoisomerase 3 beta 1, DNA topoisomerase III beta 1, TOP3B1 UniProt ID O95985 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:1000 – 1:2000 Human ICC/IF 1:100 – 1:200 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background DNA topoisomerase 3-beta-1(TOP3B), an enzyme that controls and alters the topologic states of DNA during transcription. TOP3B catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus relaxing the supercoils and altering the topology of DNA. TOP3B interacts with DNA helicase SGS1 and plays a role in DNA recombination, cellular aging and maintenance of genome stability. Low expression of TOP3B may be related to higher survival rates in breast cancer patients. Cellular location Nucleus Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: K562, 2: Human heartProtein loading amount: 20 μgExposure time: 30 secondsPredicted band size: 97 kDa Observed band size: 97 kDa ICC/IF Cell line: K-562Fixative: 4% Paraformaldehyde Permeabilization: 0.1% Triton X-100Primary Ab dilution: 1:200Primary Ab incubation condition: 4℃ overnightSecondary Ab: Goat Anti-Rabbit IgGNuclear counter stain: DAPI (blue)Counter stain: Tubulin (red)Description: The green color represents the positive signal observed with :
Recombinant Rabbit Monoclonal Anti-TOP3B Rabbit mAb Clone Number: JRMR-10725-50 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB ICC/IF Reactivity: Human Synonyms: DNA topoisomerase 3 beta 1, DNA topoisomerase III beta 1, TOP3B1 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms DNA topoisomerase 3 beta 1, DNA topoisomerase III beta 1, TOP3B1 UniProt ID O95985 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:1000 – 1:2000 Human ICC/IF 1:100 – 1:200 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background DNA topoisomerase 3-beta-1(TOP3B), an enzyme that controls and alters the topologic states of DNA during transcription. TOP3B catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus relaxing the supercoils and altering the topology of DNA. TOP3B interacts with DNA helicase SGS1 and plays a role in DNA recombination, cellular aging and maintenance of genome stability. Low expression of TOP3B may be related to higher survival rates in breast cancer patients. Cellular location Nucleus Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: K562, 2: Human heartProtein loading amount: 20 μgExposure time: 30 secondsPredicted band size: 97 kDa Observed band size: 97 kDa ICC/IF Cell line: K-562Fixative: 4% Paraformaldehyde Permeabilization: 0.1% Triton X-100Primary Ab dilution: 1:200Primary Ab incubation condition: 4℃ overnightSecondary Ab: Goat Anti-Rabbit IgGNuclear counter stain: DAPI (blue)Counter stain: Tubulin (red)Description: The green color represents the positive signal observed with