anti-th1l mouse mab

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Recombinant Anti-TH1L Mouse mAb Clone Number: 13551 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB ICC/IF Reactivity: Rat Synonyms: NELFD, TH1, NELFCD Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype Ig2a/kappa Conjugate Unconjugated Synonyms NELFD, TH1, NELFCD UniProt ID Q8IXH7 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human ICC/IF 1:50 Human Properties Purity Protein G purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. The NELF complex is involved in HIV-1 latency possibly involving recruitment of PCF11 to paused RNA polymerase II. Cellular location Nucleus Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: room temperature 2hSecondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)Lysate: HeLa, MOLT-4, A549Protein loading quantity: 20 μgExposure time: 30 sPredicted band size: 60-66 kDaObserved band size: 60-66 kDa ICC/IF Cell line: HeLaFixative: 4% ParaformaldehydePermeabilization: 0.1% TritonX-100Primary Ab dilution: 1:50Primary incubation condition: 4°C overnightSecondary Ab: Goat Anti-Mouse IgGNuclear counter stain: DAPI (Blue)Counter stain: Tubulin (Red)Description: The green color represents the positive signal observed with :
Recombinant Anti-TH1L Mouse mAb Clone Number: 13551 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB ICC/IF Reactivity: Rat Synonyms: NELFD, TH1, NELFCD Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype Ig2a/kappa Conjugate Unconjugated Synonyms NELFD, TH1, NELFCD UniProt ID Q8IXH7 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human ICC/IF 1:50 Human Properties Purity Protein G purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. The NELF complex is involved in HIV-1 latency possibly involving recruitment of PCF11 to paused RNA polymerase II. Cellular location Nucleus Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: room temperature 2hSecondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)Lysate: HeLa, MOLT-4, A549Protein loading quantity: 20 μgExposure time: 30 sPredicted band size: 60-66 kDaObserved band size: 60-66 kDa ICC/IF Cell line: HeLaFixative: 4% ParaformaldehydePermeabilization: 0.1% TritonX-100Primary Ab dilution: 1:50Primary incubation condition: 4°C overnightSecondary Ab: Goat Anti-Mouse IgGNuclear counter stain: DAPI (Blue)Counter stain: Tubulin (Red)Description: The green color represents the positive signal observed with