anti-tdp43 mouse mab

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Recombinant Anti-TDP43 Mouse mAb Clone Number: 13347 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB IHC-P ICC/IF Reactivity: Human, Mouse, Rat Synonyms: TDP43 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG1/kappa Conjugate Unconjugated Synonyms TDP43 UniProt ID Q13148 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:1000 – 1:5000 Human, Mouse IHC-P 1:50 – 1:100 Human, Mouse, Rat ICC/IF 1:50 Human Properties Purity Protein G purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background RNA-binding protein that is involved in various steps of RNA biogenesis and processing. Preferentially binds, via its two RNA recognition motifs RRM1 and RRM2, to GU-repeats on RNA molecules predominantly localized within long introns and in the 3’UTR of mRNAs. In turn, regulates the splicing of many non-coding and protein-coding RNAs including proteins involved in neuronal survival, as well as mRNAs that encode proteins relevant for neurodegenerative diseases. Plays a role in maintaining mitochondrial homeostasis by regulating the processing of mitochondrial transcripts. Regulates also mRNA stability by recruiting CNOT7/CAF1 deadenylase on mRNA 3’UTR leading to poly(A) tail deadenylation and thus shortening. In response to oxidative insult, associates with stalled ribosomes localized to stress granules (SGs) and contributes to cell survival. Participates also in the normal skeletal muscle formation and regeneration, forming cytoplasmic myo-granules and binding mRNAs that encode sarcomeric proteins. Cellular location Cytoplasm, Nucleus Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:5000Primary Ab incubation condition: 4°C overnightSecondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)Lysate: 1: HeLa, 2: Jurkat, 3: HepG2, 4: NIH/3T3Protein loading quantity: 20 μgExposure time: 60 sPredicted band size: 45 kDaObserved band size: 43 kDa IHC-P Tissue: Human spleenSection type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)Counter stain: Hematoxylin (Blue)Description: The brown color represents the positive signal observed with :
Recombinant Anti-TDP43 Mouse mAb Clone Number: 13347 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB IHC-P ICC/IF Reactivity: Human, Mouse, Rat Synonyms: TDP43 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG1/kappa Conjugate Unconjugated Synonyms TDP43 UniProt ID Q13148 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:1000 – 1:5000 Human, Mouse IHC-P 1:50 – 1:100 Human, Mouse, Rat ICC/IF 1:50 Human Properties Purity Protein G purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background RNA-binding protein that is involved in various steps of RNA biogenesis and processing. Preferentially binds, via its two RNA recognition motifs RRM1 and RRM2, to GU-repeats on RNA molecules predominantly localized within long introns and in the 3’UTR of mRNAs. In turn, regulates the splicing of many non-coding and protein-coding RNAs including proteins involved in neuronal survival, as well as mRNAs that encode proteins relevant for neurodegenerative diseases. Plays a role in maintaining mitochondrial homeostasis by regulating the processing of mitochondrial transcripts. Regulates also mRNA stability by recruiting CNOT7/CAF1 deadenylase on mRNA 3’UTR leading to poly(A) tail deadenylation and thus shortening. In response to oxidative insult, associates with stalled ribosomes localized to stress granules (SGs) and contributes to cell survival. Participates also in the normal skeletal muscle formation and regeneration, forming cytoplasmic myo-granules and binding mRNAs that encode sarcomeric proteins. Cellular location Cytoplasm, Nucleus Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:5000Primary Ab incubation condition: 4°C overnightSecondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)Lysate: 1: HeLa, 2: Jurkat, 3: HepG2, 4: NIH/3T3Protein loading quantity: 20 μgExposure time: 60 sPredicted band size: 45 kDaObserved band size: 43 kDa IHC-P Tissue: Human spleenSection type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)Counter stain: Hematoxylin (Blue)Description: The brown color represents the positive signal observed with