anti-tc10 mouse mab

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Recombinant Anti-TC10 Mouse mAb Clone Number: 11464 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB ICC/IF Reactivity: Human, Mouse Synonyms: RHOQ, ARHQ, RASL7A Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG2b/kappa Conjugate Unconjugated Synonyms RHOQ, ARHQ, RASL7A UniProt ID P17081 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Mouse ICC/IF 1:50 – 1:200 Human Properties Purity Protein G purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Rho-related GTP-binding protein RhoQ, also known as TC10 , is a protein that is encoded by the RHOQ gene in human. TC10 is a member of the RAS superfamily of small GTP-binding proteins involved in insulin-stimulated glucose uptake. In melanocytic cells RHOQ gene expression may be regulated by MITF. It is a plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses. TC10 involved in epithelial cell polarization processes, and it may play a role in CFTR trafficking to the plasma membrane. Cellular location Cytoplasm, Membrane Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate) Lysate: 1: Jurkat, 2: Mouse brain Protein loading quantity: 20 μg Exposure time: 30 secondsPredicted band size: 23 kDa Observed band size: 23 kDa ICC/IF Cell line: Jurkat Fixative: 100% Ice-cold methanol Permeabilization: 0.1% Triton X-100 Primary Ab dilution: 1:200 Primary Ab incubation condition: 4°C overnight Secondary Ab: Goat Anti-Mouse IgGNuclear counter stain: DAPI (Blue) Description: The green color represents the positive signal observed with :
Recombinant Anti-TC10 Mouse mAb Clone Number: 11464 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB ICC/IF Reactivity: Human, Mouse Synonyms: RHOQ, ARHQ, RASL7A Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG2b/kappa Conjugate Unconjugated Synonyms RHOQ, ARHQ, RASL7A UniProt ID P17081 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Mouse ICC/IF 1:50 – 1:200 Human Properties Purity Protein G purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Rho-related GTP-binding protein RhoQ, also known as TC10 , is a protein that is encoded by the RHOQ gene in human. TC10 is a member of the RAS superfamily of small GTP-binding proteins involved in insulin-stimulated glucose uptake. In melanocytic cells RHOQ gene expression may be regulated by MITF. It is a plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses. TC10 involved in epithelial cell polarization processes, and it may play a role in CFTR trafficking to the plasma membrane. Cellular location Cytoplasm, Membrane Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate) Lysate: 1: Jurkat, 2: Mouse brain Protein loading quantity: 20 μg Exposure time: 30 secondsPredicted band size: 23 kDa Observed band size: 23 kDa ICC/IF Cell line: Jurkat Fixative: 100% Ice-cold methanol Permeabilization: 0.1% Triton X-100 Primary Ab dilution: 1:200 Primary Ab incubation condition: 4°C overnight Secondary Ab: Goat Anti-Mouse IgGNuclear counter stain: DAPI (Blue) Description: The green color represents the positive signal observed with