anti-sumo-1 mouse mab  (c-terminal)

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Anti-SUMO-1 Mouse mAb (C-terminal) Clone Number: 3C5 Host: Mouse Clonality: Monoclonal Applications： WB Reactivity: Human, Mouse, Rat, Monkey Synonyms: SUMO1, small ubiquitin-related modifier 1, GAP-modifying protein 1 (GMP1), SMT3 homolog 3, Ubiquitin-like protein UBL1 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms SUMO1, small ubiquitin-related modifier 1, GAP-modifying protein 1 (GMP1), SMT3 homolog 3, Ubiquitin-like protein UBL1 UniProt ID P63165 Immunogen Synthetic peptide corresponding to the C-terminus of human SUMO-1 MW (kDa) 12 Specificity Anti-SUMO-1 Mouse mAb (C-terminal) detects endogenous levels of SUMO-1 but not SUMOylated proteins. Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Mouse, Rat, Monkey Properties Purity Protein G and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Small ubiquitin-related modifier 1, 2, and 3 (SUMO-1, 2, and 3) are members of the ubiquitin-like protein family. Similar to ubiquitin, SUMO proteins bind to target proteins through a post-translational modification system. SUMO-1 can be covalently attached to target proteins through the formation of an isopeptide bond between its C-terminal Gly97 and a lysine residue in the target protein. This process is important for regulating nuclear trafficking, formation of subnuclear structures, transcriptional activity, and protein stability, with SUMO-1 being conjugated to proteins such as RanGAP, PML, p53 and IκB-α. Cellular location Nucleus, Cytoplasm Images WB Lysate: (-): HeLa; (+): HeLa + MG-132 (10 μM, 1 hour) Protein loading amount: 20 μg Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate) Exposure time: 60 seconds Predicted band size: 12 kDa Observed band size: 18 kDa Lysate: N2a, BRL, COS-7, MCF-7, mouse liver Protein loading amount: 20 μg Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate) Exposure time: 60 seconds Predicted band size: 12 kDa Observed band size: 18 kDa :
Anti-SUMO-1 Mouse mAb (C-terminal) Clone Number: 3C5 Host: Mouse Clonality: Monoclonal Applications： WB Reactivity: Human, Mouse, Rat, Monkey Synonyms: SUMO1, small ubiquitin-related modifier 1, GAP-modifying protein 1 (GMP1), SMT3 homolog 3, Ubiquitin-like protein UBL1 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms SUMO1, small ubiquitin-related modifier 1, GAP-modifying protein 1 (GMP1), SMT3 homolog 3, Ubiquitin-like protein UBL1 UniProt ID P63165 Immunogen Synthetic peptide corresponding to the C-terminus of human SUMO-1 MW (kDa) 12 Specificity Anti-SUMO-1 Mouse mAb (C-terminal) detects endogenous levels of SUMO-1 but not SUMOylated proteins. Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Mouse, Rat, Monkey Properties Purity Protein G and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Small ubiquitin-related modifier 1, 2, and 3 (SUMO-1, 2, and 3) are members of the ubiquitin-like protein family. Similar to ubiquitin, SUMO proteins bind to target proteins through a post-translational modification system. SUMO-1 can be covalently attached to target proteins through the formation of an isopeptide bond between its C-terminal Gly97 and a lysine residue in the target protein. This process is important for regulating nuclear trafficking, formation of subnuclear structures, transcriptional activity, and protein stability, with SUMO-1 being conjugated to proteins such as RanGAP, PML, p53 and IκB-α. Cellular location Nucleus, Cytoplasm Images WB Lysate: (-): HeLa; (+): HeLa + MG-132 (10 μM, 1 hour) Protein loading amount: 20 μg Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate) Exposure time: 60 seconds Predicted band size: 12 kDa Observed band size: 18 kDa Lysate: N2a, BRL, COS-7, MCF-7, mouse liver Protein loading amount: 20 μg Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate) Exposure time: 60 seconds Predicted band size: 12 kDa Observed band size: 18 kDa