anti-suclg1 rabbit mab

Images :
WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:10000 Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: HepG2Protein loading amount: 20 μg Exposure time: 30 sPredicted MW: 36 kDaObserved MW: 26-36 kDa Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: HeLaProtein loading amount: 20 μg Exposure time: 60 sPredicted MW: 36 kDaObserved MW: 26-36 kDa Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: A431Protein loading amount: 20 μg Exposure time: 30 sPredicted MW: 36 kDaObserved MW: 26-36 kDa IHC-P Tissue: Human kidney Section type: Formalin-fixed & paraffin-embedded sectionRetrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0Primary Ab dilution: 1:1000Primary Ab incubation condition: 1 hour at room temperatureSecondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use) Counter stain: Hematoxylin (blue) Comment: Color brown is the positive signal for