anti-succinyllysine rabbit pab

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Anti-Succinyllysine Rabbit pAb Clone Number: / Host: Rabbit Clonality: Polyclonal Applications： WB IHC-P Reactivity: All Synonyms: Ksu Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms Ksu UniProt ID / Immunogen Succinylated lysine peptides MW (kDa) Multiple Specificity Anti-Succinyllysine Rabbit pAb detects proteins post-translationally modified by succinylation on lysine residues. This pan antibody recognizes succinylated lysine independent of its surrounding sequences. Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 All IHC-P 1:100 – 1:500 Human Properties Purity Protein A and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Succinylation is a post-translational modification that involves the addition of a succinyl group (-CO-CH2-CH2-CO2 h) is added to a lysine residue of a protein molecule. This modification is found in many proteins, including histones. Although the precise implications of succinylation are under investigation, due to its impact on lysine’s charge, transitioning it from +1 to -1 (under physiological pH conditions), and because succinylation introduces a relatively substantial structural moiety with a mass of 100 Da, which is notably larger than the moieties introduced by acetylation (42 Da) or methylation (14 Da), succinylation is anticipated to induce more substantial alterations in both protein structure and function. Key players in the succinylation process comprise ‘writers’ such as carnitine palmitoyltransferase 1A (CPT1A) and lysine acetyltransferase 2A (KAT2A/GCN5), ‘erasers’ like sirtuin 5 (SIRT5) and sirtuin 7 (SIRT7), and the pH-dependent reader, the YEATS domain of glioma-amplified sequence 41 (GAS41). Cellular location / Images Dot Blot Peptide amount: 4 ng, 20 ng, 100 ng Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 30 seconds The list of peptides used in the experiment is provided below.Lane 1: Succinylated peptide library. Dot 2: Malonylated peptide library. Dot 3: Glutarylated peptide library. Dot 4: Unmodified peptide library. WB Lysates: E. coli, S. cerevisiae, D. melanogaster, mouse, and human cells Protein loading amount: 20 μg Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: (A) :
Anti-Succinyllysine Rabbit pAb Clone Number: / Host: Rabbit Clonality: Polyclonal Applications： WB IHC-P Reactivity: All Synonyms: Ksu Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms Ksu UniProt ID / Immunogen Succinylated lysine peptides MW (kDa) Multiple Specificity Anti-Succinyllysine Rabbit pAb detects proteins post-translationally modified by succinylation on lysine residues. This pan antibody recognizes succinylated lysine independent of its surrounding sequences. Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 All IHC-P 1:100 – 1:500 Human Properties Purity Protein A and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Succinylation is a post-translational modification that involves the addition of a succinyl group (-CO-CH2-CH2-CO2 h) is added to a lysine residue of a protein molecule. This modification is found in many proteins, including histones. Although the precise implications of succinylation are under investigation, due to its impact on lysine’s charge, transitioning it from +1 to -1 (under physiological pH conditions), and because succinylation introduces a relatively substantial structural moiety with a mass of 100 Da, which is notably larger than the moieties introduced by acetylation (42 Da) or methylation (14 Da), succinylation is anticipated to induce more substantial alterations in both protein structure and function. Key players in the succinylation process comprise ‘writers’ such as carnitine palmitoyltransferase 1A (CPT1A) and lysine acetyltransferase 2A (KAT2A/GCN5), ‘erasers’ like sirtuin 5 (SIRT5) and sirtuin 7 (SIRT7), and the pH-dependent reader, the YEATS domain of glioma-amplified sequence 41 (GAS41). Cellular location / Images Dot Blot Peptide amount: 4 ng, 20 ng, 100 ng Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 30 seconds The list of peptides used in the experiment is provided below.Lane 1: Succinylated peptide library. Dot 2: Malonylated peptide library. Dot 3: Glutarylated peptide library. Dot 4: Unmodified peptide library. WB Lysates: E. coli, S. cerevisiae, D. melanogaster, mouse, and human cells Protein loading amount: 20 μg Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: (A)