anti-succinyl-histone h3 (lys79) rabbit pab

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Anti-Succinyl-Histone H3 (Lys79) Rabbit pAb Clone Number: / Host: Rabbit Clonality: Polyclonal Applications： WB Reactivity: Human, Mouse, Rat Synonyms: H3K79su Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H3K79su UniProt ID P68431 Immunogen Succinylated human histone H3 (Lys79) peptide MW (kDa) 15 Specificity Anti-Succinyl-Histone H3 (Lys79) Rabbit pAb detects histone H3 only when it is succinylated at Lys79. Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Mouse, Rat Properties Purity Protein A and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Succinylation is a post-translational modification where a succinyl group (-CO-CH2-CH2-COOH) is added to a lysine residue in proteins, including histones. This addition of succinyl group changes lysine’s charge from +1 to -1 (at physiological pH) and introduces a relatively large structural moiety (100 Da), bigger than acetylation (42 Da) or methylation (14 Da). It is expected to lead to more significant changes in protein structure and function. Lysine succinylation, particularly at Lys79 on histone H3, is mediated by the enzyme KAT2A and has implications in tumor growth and disease progression. KAT2A-mediated succinylation is also observed in hepatitis B virus (HBV) infection, impacting HBV replication and epigenetic regulation. Cellular location Nucleus Images Dot Blot Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Immunogen peptide quantity: 1 ng, 4 ng, 16 ngExposure time: 60 sThe list of peptides is included in the table below. WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: U87±shKAT2A, U251±shKAT2AProtein loading quantity: 20 μgExposure time: 60 sPredicted band size: 17 kDaObserved band size: 17 kDa The figure was published on the Joural of Nature(2017, DOI: 10.1038/nature25003).Note: Immunoblotting analyses were performed with the indicated antibodies. KAT2A succinylates histone H3. KAT2A-mediated histone H3 succinylation was analysed by mixing purified KAT2A, histone H3, and succinyl-CoA. Heat-inactived KAT2A was used as a negative control. :
Anti-Succinyl-Histone H3 (Lys79) Rabbit pAb Clone Number: / Host: Rabbit Clonality: Polyclonal Applications： WB Reactivity: Human, Mouse, Rat Synonyms: H3K79su Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H3K79su UniProt ID P68431 Immunogen Succinylated human histone H3 (Lys79) peptide MW (kDa) 15 Specificity Anti-Succinyl-Histone H3 (Lys79) Rabbit pAb detects histone H3 only when it is succinylated at Lys79. Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Mouse, Rat Properties Purity Protein A and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Succinylation is a post-translational modification where a succinyl group (-CO-CH2-CH2-COOH) is added to a lysine residue in proteins, including histones. This addition of succinyl group changes lysine’s charge from +1 to -1 (at physiological pH) and introduces a relatively large structural moiety (100 Da), bigger than acetylation (42 Da) or methylation (14 Da). It is expected to lead to more significant changes in protein structure and function. Lysine succinylation, particularly at Lys79 on histone H3, is mediated by the enzyme KAT2A and has implications in tumor growth and disease progression. KAT2A-mediated succinylation is also observed in hepatitis B virus (HBV) infection, impacting HBV replication and epigenetic regulation. Cellular location Nucleus Images Dot Blot Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Immunogen peptide quantity: 1 ng, 4 ng, 16 ngExposure time: 60 sThe list of peptides is included in the table below. WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: U87±shKAT2A, U251±shKAT2AProtein loading quantity: 20 μgExposure time: 60 sPredicted band size: 17 kDaObserved band size: 17 kDa The figure was published on the Joural of Nature(2017, DOI: 10.1038/nature25003).Note: Immunoblotting analyses were performed with the indicated antibodies. KAT2A succinylates histone H3. KAT2A-mediated histone H3 succinylation was analysed by mixing purified KAT2A, histone H3, and succinyl-CoA. Heat-inactived KAT2A was used as a negative control.