anti-succinyl-histone h2b (lys120) rabbit pab

Vapor Pressure :
Anti-Succinyl-Histone H2B (Lys120) Rabbit pAb Clone Number: / Host: Rabbit Clonality: Polyclonal Applications： WB Reactivity: Human, Mouse, Rat Synonyms: H2BK120su Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H2BK120su UniProt ID P62807 Immunogen Succinylated human histone H2B (Lys120) peptide MW (kDa) 14 Specificity Anti-Succinyl-Histone H2B (Lys120) Rabbit pAb detects histone H2B only when it is succinylated at Lys120. Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Mouse, Rat Properties Purity Protein A and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histones are subject to a variety of enzyme catalyzed modifications including acetylation, phosphorylation, ubiquitylation etc. Lysine succinylation is a recently identified novel protein post-translational modification (PTM). Lysine succinylation is evolutionarily conserved and abundantly present in proteins in both prokaryotic and eukaryotic cells, suggesting its roles in regulating cellular physiology. This antibody will facilitate studies of lysine succinylation biology by identifying its substrates and studying its function. Cellular location Nucleus Images Dot Blot Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Peptide quantity: 1 ng, 4 ng, 16 ng Exposure time: 60 secondsThe list of peptides is included in the table below. WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Lysate: (-) HeLa; (+) HeLa + disodium succinylate (10 mM, 4 hours) Protein loading amount: 20 μg Exposure time: 60 secondsPredicted band size: 14 kDaObserved band size: 14 kDa :
Anti-Succinyl-Histone H2B (Lys120) Rabbit pAb Clone Number: / Host: Rabbit Clonality: Polyclonal Applications： WB Reactivity: Human, Mouse, Rat Synonyms: H2BK120su Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H2BK120su UniProt ID P62807 Immunogen Succinylated human histone H2B (Lys120) peptide MW (kDa) 14 Specificity Anti-Succinyl-Histone H2B (Lys120) Rabbit pAb detects histone H2B only when it is succinylated at Lys120. Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Mouse, Rat Properties Purity Protein A and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histones are subject to a variety of enzyme catalyzed modifications including acetylation, phosphorylation, ubiquitylation etc. Lysine succinylation is a recently identified novel protein post-translational modification (PTM). Lysine succinylation is evolutionarily conserved and abundantly present in proteins in both prokaryotic and eukaryotic cells, suggesting its roles in regulating cellular physiology. This antibody will facilitate studies of lysine succinylation biology by identifying its substrates and studying its function. Cellular location Nucleus Images Dot Blot Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Peptide quantity: 1 ng, 4 ng, 16 ng Exposure time: 60 secondsThe list of peptides is included in the table below. WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Lysate: (-) HeLa; (+) HeLa + disodium succinylate (10 mM, 4 hours) Protein loading amount: 20 μg Exposure time: 60 secondsPredicted band size: 14 kDaObserved band size: 14 kDa