anti-sting rabbit mab

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Recombinant Rabbit Monoclonal Anti-STING Rabbit mAb Clone Number: JRMR-280 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P ICC/IF Reactivity: Human Synonyms: hSTING, ERIS, hMITA, TMEM173 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms hSTING, ERIS, hMITA, TMEM173 UniProt ID Q86WV6 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human IHC-P 1:200 – 1:1000 Human ICC/IF 1:50 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background STING has several transmembrane domains, and is expressed in a variety of endothelial cells, epithelial cells and hematopoietic liver cells. STING participates in the DNA-mediated innate immune response. Non-CpG double-stranded DNA from viruses or bacteria binds to cGAS protein and is catalyzed to the second messenger cGAMP; then the dimerized STING binds to cGAMP and undergoes conformational changes. Through the autophagsome and the endoplasmic reticulum and the Golgi apparatus, it further enters the cytoplasm. During the period, it is ubiquitinated and recruited TBK1 protein, then phosphorylated, and then combined with IRF3; then IRF3 is phosphorylated and enters the nucleus to induce the expression of IFNs, etc. In conclusion, it can enhance the body’s immune ability and kill bacteria, viruses and other pathogens to a greater extent. Cellular location Endoplasmic reticulum membrane, autophagosome membrane, Endoplasmic reticulum-Golgi intermediate compartment membrane Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureLysate: THP-1, Raw264.7, HL-60Protein loading quantity: 20 μgExposure time: 30 sPredicted band size: 42 kDaObserved band size: 36 kDa IHC-P Tissue: Human tonsilSection type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:1000Primary Ab incubation condition: 1 hour at room temperature Counter stain: HematoxylinDescription: The brown color represents the positive signal observed with :
Recombinant Rabbit Monoclonal Anti-STING Rabbit mAb Clone Number: JRMR-280 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P ICC/IF Reactivity: Human Synonyms: hSTING, ERIS, hMITA, TMEM173 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms hSTING, ERIS, hMITA, TMEM173 UniProt ID Q86WV6 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human IHC-P 1:200 – 1:1000 Human ICC/IF 1:50 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background STING has several transmembrane domains, and is expressed in a variety of endothelial cells, epithelial cells and hematopoietic liver cells. STING participates in the DNA-mediated innate immune response. Non-CpG double-stranded DNA from viruses or bacteria binds to cGAS protein and is catalyzed to the second messenger cGAMP; then the dimerized STING binds to cGAMP and undergoes conformational changes. Through the autophagsome and the endoplasmic reticulum and the Golgi apparatus, it further enters the cytoplasm. During the period, it is ubiquitinated and recruited TBK1 protein, then phosphorylated, and then combined with IRF3; then IRF3 is phosphorylated and enters the nucleus to induce the expression of IFNs, etc. In conclusion, it can enhance the body’s immune ability and kill bacteria, viruses and other pathogens to a greater extent. Cellular location Endoplasmic reticulum membrane, autophagosome membrane, Endoplasmic reticulum-Golgi intermediate compartment membrane Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureLysate: THP-1, Raw264.7, HL-60Protein loading quantity: 20 μgExposure time: 30 sPredicted band size: 42 kDaObserved band size: 36 kDa IHC-P Tissue: Human tonsilSection type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:1000Primary Ab incubation condition: 1 hour at room temperature Counter stain: HematoxylinDescription: The brown color represents the positive signal observed with