anti-ankrd2 rabbit pab

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Anti-ANKRD2 Rabbit pAb Clone Number: / Host: Rabbit Clonality: Polyclonal Applications： WB IHC-P Reactivity: Human, Mouse, Rat Synonyms: hArpp, ARPP Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms hArpp, ARPP UniProt ID Q9GZV1 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Mouse, Rat IHC-P 1:500 – 1:2000 Human, Mouse, Rat Properties Purity Protein A and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background ANKRD2 belongs to the conserved muscle ankyrin repeat protein (MARP) family. Expression of MARPs is induced in response to physiologic stress, injury, and hypertrophy. Together with ANKRD1/CARP and DARP, ANKRD2 are members of the MARP mechanosensing proteins that form a complex with titin (N2A)/calpain 3 protease/myopalladin. ANKRD2 is thought to have dual, structural and signaling roles, and could link the elastic I-band region as a stress sensor for transcriptional control in the nucleus. Cellular location Nucleus, Cytoplasm Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb(HRP Conjugate)Lysate: 1: Mouse skeletal muscle; 2: Rat skeletal muscleProtein loading quantity: 20 μgExposure time: 60 sPredicted band size: 40 kDaObserved band size: 40 kDa IHC-P Tissue: Human skeletal muscleSection type: Formalin-fixed & Paraffinembedded sectionRetrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0Primary Ab dilution: 1:2000Primary Ab incubation condition: 1 hour at room temperatureSecondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)Counter stain: Hematoxylin (Blue)Description: The brown color represents the positive signal observed with :
Anti-ANKRD2 Rabbit pAb Clone Number: / Host: Rabbit Clonality: Polyclonal Applications： WB IHC-P Reactivity: Human, Mouse, Rat Synonyms: hArpp, ARPP Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms hArpp, ARPP UniProt ID Q9GZV1 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Mouse, Rat IHC-P 1:500 – 1:2000 Human, Mouse, Rat Properties Purity Protein A and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background ANKRD2 belongs to the conserved muscle ankyrin repeat protein (MARP) family. Expression of MARPs is induced in response to physiologic stress, injury, and hypertrophy. Together with ANKRD1/CARP and DARP, ANKRD2 are members of the MARP mechanosensing proteins that form a complex with titin (N2A)/calpain 3 protease/myopalladin. ANKRD2 is thought to have dual, structural and signaling roles, and could link the elastic I-band region as a stress sensor for transcriptional control in the nucleus. Cellular location Nucleus, Cytoplasm Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb(HRP Conjugate)Lysate: 1: Mouse skeletal muscle; 2: Rat skeletal muscleProtein loading quantity: 20 μgExposure time: 60 sPredicted band size: 40 kDaObserved band size: 40 kDa IHC-P Tissue: Human skeletal muscleSection type: Formalin-fixed & Paraffinembedded sectionRetrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0Primary Ab dilution: 1:2000Primary Ab incubation condition: 1 hour at room temperatureSecondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)Counter stain: Hematoxylin (Blue)Description: The brown color represents the positive signal observed with