anti-stat1 rabbit mab

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Recombinant Rabbit Monoclonal Anti-STAT1 Rabbit mAb Clone Number: JRMR-10313-20 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P IP Reactivity: Human Synonyms: / Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms / UniProt ID P42225 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human IHC-P 1:200 – 1:1000 Human IP 1:25 – 1:50 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Signal transducer and activator of transcription 1 (STAT1) is an important protein that connects cell membrane receptors and effectors for signal transduction. STAT1 is activated by tyrosine kinase (JAK) and mitogen-activated protein kinases (MAPK) to phosphorylate the conserved tyrosine and serine residues in the C-terminal activation region to form a dimer After translocation enters the nucleus and regulates target genes, its main signaling pathway is dependent on the interferon (IFN) signaling pathway. STAT1 is highly expressed in normal human brain tissues, but is low or not expressed in human glioma tissues. STAT1 has an inhibitory effect on gliomas, mainly by promoting cell apoptosis, inhibiting cell proliferation, negatively regulating the cell cycle, inhibiting tumor angiogenesis, and reducing tumor migration and invasion capabilities. In some tumors, the excessive activation of STAT1 does not induce tumor cell apoptosis, but promotes tumor cell proliferation and drug resistance. Cellular location Cytoplasm, Nucleus Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: HeLa, 2: MCF-7, 3: HT-29, 4: 293TProtein loading quantity: 20 μgExposure time: 60 sPredicted band size: 87 kDaObserved band size: 91 kDa Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: MCF-7 cell lysate, the STAT1 Rabbit mAb ( :
Recombinant Rabbit Monoclonal Anti-STAT1 Rabbit mAb Clone Number: JRMR-10313-20 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P IP Reactivity: Human Synonyms: / Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms / UniProt ID P42225 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human IHC-P 1:200 – 1:1000 Human IP 1:25 – 1:50 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Signal transducer and activator of transcription 1 (STAT1) is an important protein that connects cell membrane receptors and effectors for signal transduction. STAT1 is activated by tyrosine kinase (JAK) and mitogen-activated protein kinases (MAPK) to phosphorylate the conserved tyrosine and serine residues in the C-terminal activation region to form a dimer After translocation enters the nucleus and regulates target genes, its main signaling pathway is dependent on the interferon (IFN) signaling pathway. STAT1 is highly expressed in normal human brain tissues, but is low or not expressed in human glioma tissues. STAT1 has an inhibitory effect on gliomas, mainly by promoting cell apoptosis, inhibiting cell proliferation, negatively regulating the cell cycle, inhibiting tumor angiogenesis, and reducing tumor migration and invasion capabilities. In some tumors, the excessive activation of STAT1 does not induce tumor cell apoptosis, but promotes tumor cell proliferation and drug resistance. Cellular location Cytoplasm, Nucleus Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: HeLa, 2: MCF-7, 3: HT-29, 4: 293TProtein loading quantity: 20 μgExposure time: 60 sPredicted band size: 87 kDaObserved band size: 91 kDa Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: MCF-7 cell lysate, the STAT1 Rabbit mAb (