anti-sp1 rabbit mab

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Recombinant Rabbit Monoclonal Anti-SP1 Rabbit mAb Clone Number: JMMR-3538 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P Reactivity: Human, Mouse, Rat Synonyms: Transcription factor Sp1, TSFP1 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG/kappa Conjugate Unconjugated Synonyms Transcription factor Sp1, TSFP1 UniProt ID P08047 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:1000 Human IHC-P 1:50 Human, Mouse, Rat Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Specificity protein 1 (SP1) is a ubiquitously expressed transcription factor belonging to the family of C2H2-type zinc finger containing DNA-binding proteins. SP1 binds GC-rich motifs with high affinity and regulates the expression of numerous mammalian genes. It interacts with many other transcription factors, such as c-Myc, EGR1, and Stat1, and with basal transcription machinery components. SP1 interacts with chromatin-modifying factors, such as histone deacetylases (HDACs) and p300 in chromatin remodeling. Transcriptional activity and stability of SP1 are regulated by post-translational modification, including phosphorylation, acetylation, ubiquitination, and glycosylation. Glycosylation of SP1 following insulin treatment leads to increased nuclear localization, while glucagon treatment increases cytoplasmic SP1 levels. Investigators have found high levels of SP1 in patients with Alzheimer’s disease. Cellular location Cytoplasm, Nucleus Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgGH&L (HRP Conjugate) Lysate: 1: HeLa, 2: K562 Protein loading quantity: 20 μg Exposure time: 30 secondsPredicted band size: 81 kDa Observed band size: 100 kDa IHC-P Tissue: Human colon carcinoma Section type: Formalin-fixed & Paraffin-embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary Ab dilution: 1:50 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)Counter stain: Hematoxylin (Blue) Description: The brown color represents the positive signal observed with :
Recombinant Rabbit Monoclonal Anti-SP1 Rabbit mAb Clone Number: JMMR-3538 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P Reactivity: Human, Mouse, Rat Synonyms: Transcription factor Sp1, TSFP1 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG/kappa Conjugate Unconjugated Synonyms Transcription factor Sp1, TSFP1 UniProt ID P08047 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:1000 Human IHC-P 1:50 Human, Mouse, Rat Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Specificity protein 1 (SP1) is a ubiquitously expressed transcription factor belonging to the family of C2H2-type zinc finger containing DNA-binding proteins. SP1 binds GC-rich motifs with high affinity and regulates the expression of numerous mammalian genes. It interacts with many other transcription factors, such as c-Myc, EGR1, and Stat1, and with basal transcription machinery components. SP1 interacts with chromatin-modifying factors, such as histone deacetylases (HDACs) and p300 in chromatin remodeling. Transcriptional activity and stability of SP1 are regulated by post-translational modification, including phosphorylation, acetylation, ubiquitination, and glycosylation. Glycosylation of SP1 following insulin treatment leads to increased nuclear localization, while glucagon treatment increases cytoplasmic SP1 levels. Investigators have found high levels of SP1 in patients with Alzheimer’s disease. Cellular location Cytoplasm, Nucleus Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgGH&L (HRP Conjugate) Lysate: 1: HeLa, 2: K562 Protein loading quantity: 20 μg Exposure time: 30 secondsPredicted band size: 81 kDa Observed band size: 100 kDa IHC-P Tissue: Human colon carcinoma Section type: Formalin-fixed & Paraffin-embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary Ab dilution: 1:50 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)Counter stain: Hematoxylin (Blue) Description: The brown color represents the positive signal observed with