anti-snail mouse mab

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Recombinant Anti-Snail Mouse mAb Clone Number: JMMR-1567B Host: Mouse Clonality: Recombinant Monoclonal Applications： WB Reactivity: Human Synonyms: Zinc finger protein SNAI1, SNAI1, SNAH Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG1/kappa Conjugate Unconjugated Synonyms Zinc finger protein SNAI1, SNAI1, SNAH UniProt ID O95863 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Snail is a zinc-finger transcription factor that can repress E-cadherin transcription. Downregulation of E-cadherin is associated with epithelial-mesenchymal transition during embryonic development, a process also exploited by invasive cancer cells. Indeed, loss of E-cadherin expression is correlated with the invasive properties of some tumors and there is a considerable inverse correlation between Snail and E-cadherin mRNA levels in epithelial tumor cell lines. In addition, Snail blocks the cell cycle and confers resistance to cell death. Phosphorylation of Snail by GSK-3 and PAK1 regulates its stability, cellular localization and function. Cellular location Cytoplasm, Nucleus Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 4℃ overnight Secondary Ab: Goat Anti-Mouse IgGH&L(HRP Conjugate) Lysate: 1: MCF-7(Negative), 2: HCT-116 Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 29 kDa Observed band size: 29 kDa :
Recombinant Anti-Snail Mouse mAb Clone Number: JMMR-1567B Host: Mouse Clonality: Recombinant Monoclonal Applications： WB Reactivity: Human Synonyms: Zinc finger protein SNAI1, SNAI1, SNAH Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG1/kappa Conjugate Unconjugated Synonyms Zinc finger protein SNAI1, SNAI1, SNAH UniProt ID O95863 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Snail is a zinc-finger transcription factor that can repress E-cadherin transcription. Downregulation of E-cadherin is associated with epithelial-mesenchymal transition during embryonic development, a process also exploited by invasive cancer cells. Indeed, loss of E-cadherin expression is correlated with the invasive properties of some tumors and there is a considerable inverse correlation between Snail and E-cadherin mRNA levels in epithelial tumor cell lines. In addition, Snail blocks the cell cycle and confers resistance to cell death. Phosphorylation of Snail by GSK-3 and PAK1 regulates its stability, cellular localization and function. Cellular location Cytoplasm, Nucleus Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 4℃ overnight Secondary Ab: Goat Anti-Mouse IgGH&L(HRP Conjugate) Lysate: 1: MCF-7(Negative), 2: HCT-116 Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 29 kDa Observed band size: 29 kDa